Detailed information of TA system TA029506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/PRK09907-MazE
• Location: 1987124..1987695
• Replicon: chromosome
• Accession: NC_021281
• Organism: Fusobacterium nucleatum subsp. animalis 4 8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R9RDI3
• Locus tag: HMPREF0409_RS09415
• Protein ID: WP_008798672.1
• Coordinates: 1987124..1987447 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: R9REM8
• Locus tag: HMPREF0409_RS09420
• Protein ID: WP_016340052.1
• Coordinates: 1987441..1987695 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMPREF0409_RS09380	1982262..1983242	+	981	WP_008798668.1	polyprenyl synthetase family protein	-
HMPREF0409_RS09385	1983239..1984162	+	924	WP_008798669.1	prenyltransferase	-
HMPREF0409_RS09390	1984164..1984859	+	696	WP_008798670.1	ubiquinone/menaquinone biosynthesis methyltransferase	-
HMPREF0409_RS09395	1984883..1986178	+	1296	WP_008693338.1	FAD-dependent oxidoreductase	-
HMPREF0409_RS09400	1986181..1986465	+	285	WP_005910587.1	ferredoxin family protein	-
HMPREF0409_RS09405	1986519..1986839	-	321	WP_008798671.1	type II toxin-antitoxin system YoeB family toxin	-
HMPREF0409_RS09410	1986832..1987089	-	258	WP_008701957.1	hypothetical protein	-
HMPREF0409_RS09415	1987124..1987447	-	324	WP_008798672.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HMPREF0409_RS09420	1987441..1987695	-	255	WP_016340052.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HMPREF0409_RS09425	1987702..1987893	-	192	WP_032839825.1	hypothetical protein	-
HMPREF0409_RS09430	1987955..1990021	-	2067	WP_016340053.1	elongation factor G	-
HMPREF0409_RS09435	1990108..1991577	-	1470	WP_008798674.1	N-acetylglucosamine-specific PTS transporter subunit IIBC	-
HMPREF0409_RS09440	1991819..1992286	+	468	WP_016340054.1	NfeD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-106)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12550.70 Da
• Isoelectric Point: 5.7723

>T29506 WP_008798672.1 NC_021281:c1987447-1987124 [Fusobacterium nucleatum subsp. animalis 4_8]
VVERGDIIIINFDPIKRHEQVGKRPVLVISNEKFYKIFKLAVILPITNNTKDFPFHVLLDNRTNIKGAILCEYLKTVDLE
ERKYNKVEELPEDLLEEVLEKVSLIFQ

Nucleotide

• Length: 324 bp

>T29506 NC_021281:c1987447-1987124 [Fusobacterium nucleatum subsp. animalis 4_8]
GTGGTAGAAAGAGGAGATATTATCATAATAAATTTTGACCCTATAAAAAGACATGAACAAGTAGGGAAAAGACCAGTATT
AGTTATTAGTAATGAGAAATTCTATAAAATATTTAAACTGGCAGTAATTCTTCCAATAACAAATAATACAAAAGATTTTC
CATTTCATGTACTATTAGATAATAGAACAAATATAAAGGGTGCAATACTGTGTGAGTATTTAAAAACAGTTGATTTAGAA
GAAAGAAAATATAATAAAGTTGAAGAATTACCAGAAGATTTATTAGAAGAAGTCCTTGAAAAAGTGAGTTTAATTTTTCA
ATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 10005.53 Da
• Isoelectric Point: 6.3520

>AT29506 WP_016340052.1 NC_021281:c1987695-1987441 [Fusobacterium nucleatum subsp. animalis 4_8]
MEVLRVQKWGNSQGIRLPKKILKDLGININDKIEISLNGEEIILKKIKKYTNLDDLFKDYKGDYRKDFDEFEFFGEAQGC
EFKW

Nucleotide

• Length: 255 bp

>AT29506 NC_021281:c1987695-1987441 [Fusobacterium nucleatum subsp. animalis 4_8]
ATGGAAGTATTAAGAGTTCAAAAATGGGGAAATAGTCAAGGAATTAGATTACCTAAGAAAATTTTAAAAGACTTAGGAAT
AAATATAAATGATAAAATTGAAATAAGTTTAAATGGAGAAGAAATTATATTAAAAAAAATAAAAAAATATACTAATTTAG
ATGATTTGTTTAAAGACTATAAAGGAGATTATAGAAAAGATTTTGATGAATTTGAATTTTTTGGAGAAGCACAAGGTTGT
GAGTTTAAGTGGTAG

Structures

Toxin

• AlphaFold DB: R9RDI3

Antitoxin

• AlphaFold DB: R9REM8