Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5222224..5222849 Replicon chromosome
Accession NZ_OV753987
Organism Klebsiella pneumoniae isolate BB1542

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag O0552_RS25400 Protein ID WP_002882817.1
Coordinates 5222224..5222607 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag O0552_RS25405 Protein ID WP_004150355.1
Coordinates 5222607..5222849 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O0552_RS25385 (VKR_04995) 5219590..5220492 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
O0552_RS25390 (VKR_04996) 5220489..5221124 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
O0552_RS25395 (VKR_04997) 5221121..5222050 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
O0552_RS25400 (VKR_04998) 5222224..5222607 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
O0552_RS25405 (VKR_04999) 5222607..5222849 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
O0552_RS25410 (VKR_05000) 5223054..5223971 + 918 WP_004187175.1 alpha/beta hydrolase -
O0552_RS25415 (VKR_05001) 5223985..5224926 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
O0552_RS25420 (VKR_05002) 5224971..5225408 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
O0552_RS25425 (VKR_05003) 5225405..5226265 - 861 WP_019725422.1 virulence factor BrkB family protein -
O0552_RS25430 (VKR_05004) 5226259..5226858 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T295051 WP_002882817.1 NZ_OV753987:c5222607-5222224 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT295051 WP_004150355.1 NZ_OV753987:c5222849-5222607 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References