Detailed information of TA system TA029503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 8582943..8583962
• Replicon: chromosome
• Accession: NC_021252
• Organism: Amycolatopsis orientalis HCCB10007

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: AORI_RS79890
• Protein ID: WP_016338024.1
• Coordinates: 8582943..8583503 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: R4TJI8
• Locus tag: AORI_RS79895
• Protein ID: WP_016338025.1
• Coordinates: 8583507..8583962 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AORI_RS79865	8579207..8579770	+	564	WP_016338019.1	hypothetical protein	-
AORI_RS79870	8579767..8580714	+	948	WP_016338020.1	asparaginase	-
AORI_RS79875	8580711..8581610	-	900	WP_016338021.1	hypothetical protein	-
AORI_RS79880	8581818..8582003	+	186	WP_016338022.1	DUF3073 domain-containing protein	-
AORI_RS79885	8582092..8582952	+	861	WP_016338023.1	3-hydroxybutyryl-CoA dehydrogenase	-
AORI_RS79890	8582943..8583503	-	561	WP_016338024.1	PIN domain-containing protein	Toxin
AORI_RS79895	8583507..8583962	-	456	WP_016338025.1	helix-turn-helix domain-containing protein	Antitoxin
AORI_RS79900	8584086..8584985	+	900	WP_016338026.1	Gfo/Idh/MocA family oxidoreductase	-
AORI_RS79905	8585208..8585861	+	654	WP_016338027.1	VanZ family protein	-
AORI_RS79910	8586040..8586798	+	759	WP_016338028.1	GNAT family N-acetyltransferase	-
AORI_RS79915	8586787..8587206	-	420	WP_037334732.1	aminoacyl-tRNA hydrolase	-
AORI_RS79920	8587254..8588324	-	1071	WP_007032555.1	phosphoribosylformylglycinamidine cyclo-ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-111)

Antitoxin

• SinI-like, DNA-binding domain (82-128)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20908.98 Da
• Isoelectric Point: 5.8449

>T29503 WP_016338024.1 NC_021252:c8583503-8582943 [Amycolatopsis keratiniphila]
VAFVVLFDANVLYPNVLRDLLLRIAQSGFVQAKWTDEILDEVFRNLRKNRPDLDPGKLDRTRHLMNASVRDCLVTGYEPL
IATLDLPDPDDRHVLAAAIKAQARVIVTENLRDFPGRALGPWNVEAQTSDDFVLDRIELCERVVVEAVRRIVNSRRAPPT
SVSEVLDALERCGLRKSVAALRSGQE

Nucleotide

• Length: 561 bp

>T29503 NC_021252:c8583503-8582943 [Amycolatopsis keratiniphila]
GTGGCCTTCGTGGTCCTGTTCGACGCCAACGTGCTGTACCCCAACGTTCTACGCGATCTCCTGCTCCGGATCGCGCAGAG
CGGTTTCGTCCAGGCCAAATGGACGGACGAGATCCTCGATGAGGTTTTCCGGAACCTCCGCAAGAACCGGCCCGATCTCG
ACCCCGGCAAGCTGGACAGGACGCGGCACCTGATGAACGCCTCGGTCCGGGATTGTCTCGTCACCGGCTACGAACCGCTC
ATCGCCACGCTCGACCTGCCCGACCCGGACGACCGCCATGTCCTCGCGGCGGCCATCAAGGCGCAGGCGCGTGTCATCGT
GACCGAGAACCTGCGGGATTTTCCCGGGCGGGCCCTCGGTCCCTGGAACGTCGAGGCGCAGACCTCGGACGACTTCGTCC
TCGATCGGATCGAGCTCTGCGAACGAGTGGTCGTGGAGGCGGTGCGGCGAATCGTGAACTCACGCCGCGCTCCGCCTACC
AGCGTTTCCGAGGTTCTCGACGCTTTGGAGCGCTGCGGCCTCCGTAAATCGGTCGCGGCGCTCCGTTCGGGTCAGGAGTA
G

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16809.09 Da
• Isoelectric Point: 5.8187

>AT29503 WP_016338025.1 NC_021252:c8583962-8583507 [Amycolatopsis keratiniphila]
MTALNIDQIVPGRHDIDVALEALPHVEAYLARRHERDTVRVVVDEHEESVVVPRGVVDLMARVLALVAAGESVAIVPSHA
ELTTQQAADLLNVSRPFLIGLLEAGRIEYRTVGKHRRVKASSLHDYLREDDHRRREAADELSALNQEMGLT

Nucleotide

• Length: 456 bp

>AT29503 NC_021252:c8583962-8583507 [Amycolatopsis keratiniphila]
ATGACTGCGCTGAACATTGACCAGATCGTCCCAGGACGCCACGACATCGACGTGGCGCTCGAGGCACTCCCGCACGTCGA
GGCCTACCTGGCTCGGCGGCATGAAAGGGACACCGTGCGCGTCGTCGTCGACGAACATGAGGAGTCCGTGGTCGTCCCTC
GCGGGGTGGTCGACCTGATGGCTCGGGTGCTCGCCTTGGTGGCGGCGGGTGAGAGCGTGGCCATCGTCCCGTCCCATGCC
GAGCTGACCACACAGCAGGCGGCCGACCTGCTCAACGTCTCCCGGCCGTTCCTCATCGGGCTGCTCGAAGCGGGGCGGAT
CGAGTACCGCACGGTCGGTAAGCACCGGCGGGTGAAAGCCAGTTCGCTCCACGACTACTTGCGTGAAGACGACCATCGGC
GCCGCGAGGCCGCGGACGAGCTTTCCGCCTTGAATCAGGAGATGGGGCTGACCTAG

Structures

Antitoxin

• AlphaFold DB: R4TJI8