Detailed information of TA system TA029491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3065368..3066055
• Replicon: chromosome
• Accession: NC_021251
• Organism: Mycobacterium tuberculosis CCDC5079

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: CFBS_RS14590
• Protein ID: WP_003414064.1
• Coordinates: 3065660..3066055 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: CFBS_RS14585
• Protein ID: WP_003414061.1
• Coordinates: 3065368..3065634 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBS_RS14550	3060619..3061371	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
CFBS_RS14555	3061615..3061890	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
CFBS_RS14560	3061887..3063392	-	1506	Protein_2839	type I restriction-modification system subunit M	-
CFBS_RS14575	3064653..3064868	-	216	WP_016330425.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
CFBS_RS14580	3064955..3065371	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
CFBS_RS14585	3065368..3065634	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CFBS_RS14590	3065660..3066055	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
CFBS_RS14595	3066052..3066321	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
CFBS_RS14600	3066331..3067425	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
CFBS_RS14605	3067422..3067841	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
CFBS_RS14610	3067915..3068394	-	480	WP_003414073.1	dihydrofolate reductase	-
CFBS_RS14615	3068465..3069265	-	801	WP_003911953.1	thymidylate synthase	-
CFBS_RS14620	3069421..3070158	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3053281..3069265	15984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T29491 WP_003414064.1 NC_021251:c3066055-3065660 [Mycobacterium tuberculosis CCDC5079]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T29491 NC_021251:c3066055-3065660 [Mycobacterium tuberculosis CCDC5079]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT29491 WP_003414061.1 NC_021251:c3065634-3065368 [Mycobacterium tuberculosis CCDC5079]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT29491 NC_021251:c3065634-3065368 [Mycobacterium tuberculosis CCDC5079]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64