Detailed information of TA system TA294888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2877019..2877628
• Replicon: chromosome
• Accession: NZ_OU745390
• Organism: Pseudomonas sp. Marseille-Q3773

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LG386_RS13330
• Protein ID: WP_225778761.1
• Coordinates: 2877019..2877321 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LG386_RS13335
• Protein ID: WP_186688533.1
• Coordinates: 2877314..2877628 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG386_RS25660	2872874..2873002	-	129	WP_009683677.1	PA1414 family protein	-
LG386_RS13310	2873127..2874020	-	894	WP_225778757.1	LysR family transcriptional regulator	-
LG386_RS13315	2874126..2875295	+	1170	WP_225778758.1	MFS transporter	-
LG386_RS13320	2875423..2876349	-	927	WP_225778759.1	DMT family transporter	-
LG386_RS13325	2876456..2876851	-	396	WP_225778760.1	transcriptional regulator	-
LG386_RS13330	2877019..2877321	+	303	WP_225778761.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG386_RS13335	2877314..2877628	+	315	WP_186688533.1	putative addiction module antidote protein	Antitoxin
LG386_RS13340	2877680..2877952	+	273	WP_225778762.1	hypothetical protein	-
LG386_RS13345	2878124..2879446	-	1323	WP_225778763.1	DEAD/DEAH box helicase	-
LG386_RS13350	2879588..2880889	+	1302	WP_225778764.1	mechanosensitive ion channel family protein	-
LG386_RS13355	2880934..2881230	-	297	WP_225778765.1	DUF3077 domain-containing protein	-
LG386_RS13360	2881327..2881569	-	243	WP_225778766.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2869162..2879446	10284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (6-94)

Antitoxin

• Uncharacterized protein HI1420 (8-97)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11316.93 Da
• Isoelectric Point: 10.8773

>T294888 WP_225778761.1 NZ_OU745390:2877019-2877321 [Pseudomonas sp. Marseille-Q3773]
MKKIESSSFRHWVTGLRDANARARIISRINRLMEGLPGDVSPVGHGVSELRVHYGPGYRVYFHQAGNTFVILLCGGDKGS
QQRDIKAAHQILRSWRMQND

Nucleotide

• Length: 303 bp

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11306.83 Da
• Isoelectric Point: 6.2193

>AT294888 WP_186688533.1 NZ_OU745390:2877314-2877628 [Pseudomonas sp. Marseille-Q3773]
MTETFSEFDPAQYLTTPEAIAQFMTDALETGDAGYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMTAQPHLAGHRSRPLN

Nucleotide

• Length: 315 bp