Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4792911..4793408 Replicon chromosome
Accession NZ_OU701452
Organism Escherichia coli isolate CNR65D6

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LWS60_RS23000 Protein ID WP_001173343.1
Coordinates 4793235..4793408 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LWS60_RS22995 Protein ID WP_000398619.1
Coordinates 4792911..4793207 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS60_RS22975 (4789641) 4789641..4790360 - 720 WP_000611288.1 two-component system response regulator DcuR -
LWS60_RS22980 (4790357) 4790357..4791988 - 1632 WP_001216477.1 sensor histidine kinase -
LWS60_RS22985 (4792168) 4792168..4792398 + 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
LWS60_RS22990 (4792410) 4792410..4792682 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
LWS60_RS22995 (4792911) 4792911..4793207 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LWS60_RS23000 (4793235) 4793235..4793408 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LWS60_RS23005 (4793527) 4793527..4795044 - 1518 WP_001550472.1 lysine--tRNA ligase -
LWS60_RS23010 (4795281) 4795281..4796738 - 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T294879 WP_001173343.1 NZ_OU701452:4793235-4793408 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT294879 WP_000398619.1 NZ_OU701452:4792911-4793207 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References