Detailed information of TA system TA029486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2863137..2863768
• Replicon: chromosome
• Accession: NC_021251
• Organism: Mycobacterium tuberculosis CCDC5079

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: CFBS_RS13495
• Protein ID: WP_003413174.1
• Coordinates: 2863391..2863768 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: CFBS_RS13490
• Protein ID: WP_003413167.1
• Coordinates: 2863137..2863394 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBS_RS13465	2859052..2859366	+	315	WP_009937839.1	hypothetical protein	-
CFBS_RS13470	2859662..2860873	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
CFBS_RS13475	2861000..2861659	+	660	WP_003902296.1	hypothetical protein	-
CFBS_RS13480	2861656..2862318	+	663	WP_003905878.1	hypothetical protein	-
CFBS_RS22315	2862315..2862592	+	278	Protein_2622	type II toxin-antitoxin system VapB family antitoxin	-
CFBS_RS13485	2862685..2863098	+	414	WP_003413164.1	PIN domain nuclease	-
CFBS_RS13490	2863137..2863394	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
CFBS_RS13495	2863391..2863768	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
CFBS_RS13500	2863784..2864158	-	375	WP_003413177.1	hypothetical protein	-
CFBS_RS13505	2864258..2864653	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
CFBS_RS13510	2864650..2864895	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
CFBS_RS13515	2865306..2865725	-	420	WP_003413190.1	A24 family peptidase	-
CFBS_RS13520	2865737..2866546	-	810	WP_003413193.1	shikimate dehydrogenase	-
CFBS_RS13525	2866543..2867799	-	1257	WP_014585306.1	endolytic transglycosylase MltG	-
CFBS_RS13530	2867792..2868304	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T29486 WP_003413174.1 NC_021251:2863391-2863768 [Mycobacterium tuberculosis CCDC5079]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T29486 NC_021251:2863391-2863768 [Mycobacterium tuberculosis CCDC5079]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT29486 WP_003413167.1 NC_021251:2863137-2863394 [Mycobacterium tuberculosis CCDC5079]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT29486 NC_021251:2863137-2863394 [Mycobacterium tuberculosis CCDC5079]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5