Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1211134..1211918 Replicon chromosome
Accession NZ_OU701452
Organism Escherichia coli isolate CNR65D6

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag LWS60_RS05660 Protein ID WP_000613626.1
Coordinates 1211134..1211628 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag LWS60_RS05665 Protein ID WP_001110447.1
Coordinates 1211625..1211918 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS60_RS05640 (1206327) 1206327..1207424 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
LWS60_RS05645 (1207424) 1207424..1208365 + 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
LWS60_RS05650 (1208431) 1208431..1210074 + 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
LWS60_RS05655 (1210086) 1210086..1211039 + 954 WP_001212768.1 flagellar hook-associated protein FlgL -
LWS60_RS05660 (1211134) 1211134..1211628 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
LWS60_RS05665 (1211625) 1211625..1211918 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
LWS60_RS05670 (1212051) 1212051..1215236 - 3186 WP_001550951.1 ribonuclease E -
LWS60_RS05675 (1215809) 1215809..1216768 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T294857 WP_000613626.1 NZ_OU701452:c1211628-1211134 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT294857 WP_001110447.1 NZ_OU701452:c1211918-1211625 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References