Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 504195..504813 Replicon chromosome
Accession NZ_OU701452
Organism Escherichia coli isolate CNR65D6

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LWS60_RS02330 Protein ID WP_001291435.1
Coordinates 504195..504413 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LWS60_RS02335 Protein ID WP_000344800.1
Coordinates 504439..504813 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS60_RS02295 (499484) 499484..500056 + 573 WP_000779831.1 YbaY family lipoprotein -
LWS60_RS02300 (500087) 500087..500398 - 312 WP_000409911.1 MGMT family protein -
LWS60_RS02310 (500777) 500777..501130 + 354 WP_001550741.1 DUF1428 family protein -
LWS60_RS02315 (501172) 501172..502722 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LWS60_RS02320 (502886) 502886..503356 - 471 WP_000136192.1 YlaC family protein -
LWS60_RS02325 (503472) 503472..504023 - 552 WP_000102564.1 maltose O-acetyltransferase -
LWS60_RS02330 (504195) 504195..504413 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LWS60_RS02335 (504439) 504439..504813 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LWS60_RS02340 (505359) 505359..508508 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LWS60_RS02345 (508531) 508531..509724 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T294855 WP_001291435.1 NZ_OU701452:c504413-504195 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT294855 WP_000344800.1 NZ_OU701452:c504813-504439 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References