Detailed information of TA system TA294847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3986410..3988099
• Replicon: chromosome
• Accession: NZ_OU701449
• Organism: Escherichia coli isolate CNR85I8

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: LWS57_RS19220
• Protein ID: WP_000365809.1
• Coordinates: 3986410..3987330 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LWS57_RS19225
• Protein ID: WP_001550661.1
• Coordinates: 3987317..3988099 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LWS57_RS19205 (3982500)	3982500..3983627	-	1128	WP_001550663.1	MFS transporter	-
LWS57_RS19210 (3983769)	3983769..3984827	-	1059	WP_122987197.1	alpha/beta hydrolase	-
LWS57_RS19215 (3985092)	3985092..3985976	+	885	WP_001119037.1	LysR family transcriptional regulator	-
LWS57_RS19220 (3986410)	3986410..3987330	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LWS57_RS19225 (3987317)	3987317..3988099	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LWS57_RS19230 (3988415)	3988415..3991624	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
LWS57_RS19235 (3991971)	3991971..3992441	+	471	WP_000201185.1	hypothetical protein	-
LWS57_RS19240 (3992438)	3992438..3992540	+	103	Protein_3782	helix-turn-helix domain-containing protein	-
LWS57_RS19245 (3992632)	3992632..3992790	+	159	Protein_3783	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T294847 WP_000365809.1 NZ_OU701449:c3987330-3986410 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT294847 WP_001550661.1 NZ_OU701449:c3988099-3987317 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: V0Z9M0