Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3113855..3114639 Replicon chromosome
Accession NZ_OU701449
Organism Escherichia coli isolate CNR85I8

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag LWS57_RS15205 Protein ID WP_000613626.1
Coordinates 3114145..3114639 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag LWS57_RS15200 Protein ID WP_001110447.1
Coordinates 3113855..3114148 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS57_RS15190 (3109005) 3109005..3109964 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
LWS57_RS15195 (3110537) 3110537..3113722 + 3186 WP_001550951.1 ribonuclease E -
LWS57_RS15200 (3113855) 3113855..3114148 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
LWS57_RS15205 (3114145) 3114145..3114639 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
LWS57_RS15210 (3114734) 3114734..3115687 - 954 WP_166742443.1 flagellar hook-associated protein FlgL -
LWS57_RS15215 (3115699) 3115699..3117342 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
LWS57_RS15220 (3117408) 3117408..3118349 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
LWS57_RS15225 (3118349) 3118349..3119446 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T294844 WP_000613626.1 NZ_OU701449:3114145-3114639 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT294844 WP_001110447.1 NZ_OU701449:3113855-3114148 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References