Detailed information of TA system TA294819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 73415..75143
• Replicon: plasmid 3
• Accession: NZ_OU659164
• Organism: Providencia alcalifaciens strain 2019-04-29292-1-3 isolate 2019-04-29292-1-3

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: LDO74_RS20445
• Protein ID: WP_224051181.1
• Coordinates: 74199..75143 (+)
• Length: 315 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LDO74_RS20440
• Protein ID: WP_224051182.1
• Coordinates: 73415..74185 (+)
• Length: 257 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO74_RS20410 (NVI2019_OGMBKCAO_04104)	68419..69213	+	795	WP_224051186.1	DUF1460 domain-containing protein	-
LDO74_RS20420 (NVI2019_OGMBKCAO_04106)	69805..70491	+	687	WP_282565833.1	hypothetical protein	-
LDO74_RS20425 (NVI2019_OGMBKCAO_04107)	70464..72410	+	1947	WP_282563880.1	hypothetical protein	-
LDO74_RS20430	72538..72648	-	111	Protein_65	resolvase	-
LDO74_RS20435	72616..73125	+	510	Protein_66	Tn3 family transposase	-
LDO74_RS20440 (NVI2019_OGMBKCAO_04108)	73415..74185	+	771	WP_224051182.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LDO74_RS20445 (NVI2019_OGMBKCAO_04109)	74199..75143	+	945	WP_224051181.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LDO74_RS20450 (NVI2019_OGMBKCAO_04111)	75772..76059	-	288	WP_224051180.1	hypothetical protein	-
LDO74_RS21290	76800..76922	+	123	WP_263422652.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91748	91748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (20-277)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-97)

Sequences

Toxin

Protein

• Length: 315 a.a.
• Molecular weight: 35625.02 Da
• Isoelectric Point: 4.9792

>T294819 WP_224051181.1 NZ_OU659164:74199-75143 [Providencia alcalifaciens]
MDRDSPYYKQVSLLIKILPVIATEPTFALKGGTAINLFVRNFPRLSVDIDLAYLPLESRNDALVNVKASLLRITDLINTQ
VDTRAVLQDHKVDELRIIVNSPGGTIKVEVSPVARGALHQPSILSVQPSVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLAEEGISRDIFVGFLTYVLSHPRPINEVMDPNWKPLDEIFQTEFDGMTLVNIELEDLIEVRSAMMTALQA
HFTEKDRVFLLSFKQGNPDWALFDYPSAEGLPAIRWKLQNIAQLAKNRDKYTEQLSKLEVVLDRWLHSADKQRL

Nucleotide

• Length: 945 bp

Antitoxin

Protein

• Length: 257 a.a.
• Molecular weight: 29452.77 Da
• Isoelectric Point: 9.6991

>AT294819 WP_224051182.1 NZ_OU659164:73415-74185 [Providencia alcalifaciens]
MSSKINWLVTHTYPGSIVLQQWLTKNGISYSLAQKYTQSGWLKKLSSGVYFRPDGKDGTKPDWVDALQAINIQQCLPVHL
AGLSSLNHQGLSHYLQLRKEQLWIGIKSKQSLPKWFREFPNQDWFYCGNSKLQIDPDMDLKFITVKGKELLVSRSELAAY
EVVDAIGKQISFEHVAELFQGLVNLSPKRVQSLLERSHAIQTNRIFLFLSHHCDHQWGEYLNEKNIELGSGKRQVVNKGR
LDERYQITVPESLKTK

Nucleotide

• Length: 771 bp