Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4707196..4708819 Replicon chromosome
Accession NZ_OU453210
Organism Klebsiella pneumoniae isolate Klebsiella pneumoniae

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag K3775_RS23035 Protein ID WP_000088046.1
Coordinates 4707196..4708521 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag K3775_RS23040 Protein ID WP_024136327.1
Coordinates 4708526..4708819 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3775_RS22995 (4702833) 4702833..4703255 - 423 WP_114213117.1 organomercurial transporter MerC -
K3775_RS23000 (4703291) 4703291..4703566 - 276 WP_065203552.1 mercury resistance system periplasmic binding protein MerP -
K3775_RS23005 (4703580) 4703580..4703930 - 351 WP_001294663.1 mercuric transport protein MerT -
K3775_RS23010 (4704002) 4704002..4704436 + 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
K3775_RS23020 (4705589) 4705589..4706404 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
K3775_RS23025 (4706417) 4706417..4706827 - 411 WP_000985911.1 hypothetical protein -
K3775_RS23030 (4706929) 4706929..4707135 - 207 WP_000134171.1 hypothetical protein -
K3775_RS23035 (4707196) 4707196..4708521 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
K3775_RS23040 (4708526) 4708526..4708819 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
K3775_RS23045 (4709423) 4709423..4709815 - 393 WP_000341066.1 hypothetical protein -
K3775_RS23050 (4710188) 4710188..4710529 + 342 WP_001151575.1 hypothetical protein -
K3775_RS23055 (4710621) 4710621..4710812 + 192 WP_000398480.1 hypothetical protein -
K3775_RS23060 (4710862) 4710862..4711143 + 282 WP_000077926.1 hypothetical protein -
K3775_RS27085 (4711484) 4711484..4711615 - 132 WP_000374059.1 hypothetical protein -
K3775_RS23065 (4711706) 4711706..4712947 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element mcr-1.1 / catA1 / aph(3')-Ia - 4670546..4870323 199777


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T294768 WP_000088046.1 NZ_OU453210:c4708521-4707196 [Klebsiella pneumoniae]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT294768 WP_024136327.1 NZ_OU453210:c4708819-4708526 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References