Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4624232..4625042 Replicon chromosome
Accession NZ_OU453210
Organism Klebsiella pneumoniae isolate Klebsiella pneumoniae

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K3775_RS22625 Protein ID WP_002887280.1
Coordinates 4624232..4624765 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K3775_RS22630 Protein ID WP_002887278.1
Coordinates 4624776..4625042 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3775_RS22620 (4623064) 4623064..4624185 + 1122 WP_009309849.1 cupin domain-containing protein -
K3775_RS22625 (4624232) 4624232..4624765 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K3775_RS22630 (4624776) 4624776..4625042 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K3775_RS22635 (4625145) 4625145..4626578 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
K3775_RS22640 (4626568) 4626568..4627251 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
K3775_RS22645 (4627424) 4627424..4628809 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
K3775_RS22650 (4628827) 4628827..4629132 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T294767 WP_002887280.1 NZ_OU453210:c4624765-4624232 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT294767 WP_002887278.1 NZ_OU453210:c4625042-4624776 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References