294754

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 38893..39696 Replicon plasmid 2
Accession NZ_OU342920
Organism Escherichia coli isolate UR21/11-869

Toxin (Protein)


Gene name KacT Uniprot ID A0A0K3X5E3
Locus tag LIX80_RS24330 Protein ID WP_000348884.1
Coordinates 38893..39423 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag LIX80_RS24335 Protein ID WP_001275013.1
Coordinates 39427..39696 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIX80_RS24300 (34146) 34146..35099 + 954 WP_001068902.1 ferric citrate uptake sigma factor regulator FecR -
LIX80_RS24305 (35186) 35186..37510 + 2325 WP_021534628.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
LIX80_RS24310 (37555) 37555..37642 + 88 Protein_50 hypothetical protein -
LIX80_RS24315 (37713) 37713..37985 + 273 WP_000019163.1 hypothetical protein -
LIX80_RS24320 (37966) 37966..38616 + 651 Protein_52 transposase zinc-binding domain-containing protein -
LIX80_RS24325 (38675) 38675..38892 - 218 Protein_53 transposase -
LIX80_RS24330 (38893) 38893..39423 - 531 WP_000348884.1 GNAT family N-acetyltransferase Toxin
LIX80_RS24335 (39427) 39427..39696 - 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
LIX80_RS24340 (40584) 40584..41574 + 991 Protein_56 RepB family plasmid replication initiator protein -
LIX80_RS24345 (41857) 41857..42597 - 741 WP_021534629.1 tyrosine-type recombinase/integrase -
LIX80_RS24350 (42718) 42718..42852 - 135 Protein_58 serine/threonine protein kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..66803 66803
- flank IS/Tn - - 37966..38331 365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20320.22 Da        Isoelectric Point: 6.7486

>T294754 WP_000348884.1 NZ_OU342920:c39423-38893 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT294754 WP_001275013.1 NZ_OU342920:c39696-39427 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3X5E3

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References