Detailed information of TA system TA029472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2182339..2183042
• Replicon: chromosome
• Accession: NC_021251
• Organism: Mycobacterium tuberculosis CCDC5079

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CFBS_RS10145
• Protein ID: WP_003409778.1
• Coordinates: 2182339..2182668 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CFBS_RS21385
• Protein ID: WP_003409780.1
• Coordinates: 2182665..2183042 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBS_RS10125	2178722..2179792	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
CFBS_RS10130	2179789..2180304	+	516	WP_003409718.1	flavin reductase family protein	-
CFBS_RS10135	2180301..2181362	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CFBS_RS10140	2181359..2182129	+	771	WP_003409775.1	SDR family oxidoreductase	-
CFBS_RS10145	2182339..2182668	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CFBS_RS21385	2182665..2183042	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CFBS_RS10155	2183039..2183629	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CFBS_RS10160	2183684..2185048	+	1365	WP_003903691.1	HNH endonuclease	-
CFBS_RS10165	2185203..2185655	-	453	WP_003899095.1	lipoprotein	-
CFBS_RS10170	2185719..2186120	+	402	WP_003409869.1	hypothetical protein	-
CFBS_RS10175	2186113..2186295	-	183	WP_003409870.1	hypothetical protein	-
CFBS_RS10180	2186409..2186759	-	351	WP_003409871.1	hypothetical protein	-
CFBS_RS10185	2186770..2187672	-	903	WP_003899097.1	hypothetical protein	-
CFBS_RS10190	2187693..2187884	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T29472 WP_003409778.1 NC_021251:c2182668-2182339 [Mycobacterium tuberculosis CCDC5079]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T29472 NC_021251:c2182668-2182339 [Mycobacterium tuberculosis CCDC5079]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT29472 WP_003409780.1 NC_021251:c2183042-2182665 [Mycobacterium tuberculosis CCDC5079]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT29472 NC_021251:c2183042-2182665 [Mycobacterium tuberculosis CCDC5079]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8