Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 21620..22757 Replicon plasmid pl2
Accession NZ_OU016039
Organism Enterococcus faecium isolate USZ_VRE53_P46

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag KKQ98_RS15840 Protein ID WP_002332783.1
Coordinates 21620..22483 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag KKQ98_RS15845 Protein ID WP_002326825.1
Coordinates 22485..22757 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKQ98_RS15805 17009..17917 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
KKQ98_RS15810 17950..18684 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
KKQ98_RS15815 18665..19534 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein -
KKQ98_RS15820 19549..19773 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator -
KKQ98_RS15825 19916..20107 - 192 Protein_25 recombinase family protein -
KKQ98_RS15830 20162..20842 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
KKQ98_RS15835 20917..21057 - 141 Protein_27 IS6 family transposase -
KKQ98_RS15840 21620..22483 - 864 WP_002332783.1 zeta toxin family protein Toxin
KKQ98_RS15845 22485..22757 - 273 WP_002326825.1 antitoxin Antitoxin
KKQ98_RS15850 22775..22990 - 216 WP_001835296.1 peptide-binding protein -
KKQ98_RS15855 23082..23978 - 897 WP_002326827.1 ParA family protein -
KKQ98_RS15865 25500..25760 - 261 Protein_33 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
KKQ98_RS15870 25930..26667 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / erm(B) / cat(pC221) / VanHAX - 1..47525 47525
- inside IScluster/Tn ant(6)-Ia / erm(B) / cat(pC221) - 11377..32052 20675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T294707 WP_002332783.1 NZ_OU016039:c22483-21620 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT294707 WP_002326825.1 NZ_OU016039:c22757-22485 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References