294701

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 44245..45382 Replicon plasmid pl2
Accession NZ_OU016029
Organism Enterococcus faecium isolate USZ_VRE67_P60

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag KKQ96_RS15350 Protein ID WP_002332783.1
Coordinates 44519..45382 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag KKQ96_RS15345 Protein ID WP_002326825.1
Coordinates 44245..44517 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKQ96_RS15320 40335..41072 + 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
KKQ96_RS15325 41242..41502 + 261 Protein_47 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
KKQ96_RS15330 41573..42912 + 1340 WP_086953915.1 IS3-like element ISEnfa3 family transposase -
KKQ96_RS15335 43024..43920 + 897 WP_002326827.1 ParA family protein -
KKQ96_RS15340 44012..44227 + 216 WP_001835296.1 peptide-binding protein -
KKQ96_RS15345 44245..44517 + 273 WP_002326825.1 antitoxin Antitoxin
KKQ96_RS15350 44519..45382 + 864 WP_002332783.1 zeta toxin family protein Toxin
KKQ96_RS15355 45945..46085 + 141 Protein_53 IS6 family transposase -
KKQ96_RS15360 46160..46840 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
KKQ96_RS15365 46895..47086 + 192 Protein_55 recombinase family protein -
KKQ96_RS15370 47229..47453 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / VanHAX / cat(pC221) / erm(B) - 1..47525 47525
- inside IScluster/Tn cat(pC221) / erm(B) - 34950..46840 11890


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T294701 WP_002332783.1 NZ_OU016029:44519-45382 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT294701 WP_002326825.1 NZ_OU016029:44245-44517 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References