Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 13624..14732 Replicon plasmid pl2
Accession NZ_OU015948
Organism Enterococcus faecium isolate USZ_VRE5_P5

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag KKQ94_RS15085 Protein ID WP_000233000.1
Coordinates 13863..14732 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag KKQ94_RS15080 Protein ID WP_000205227.1
Coordinates 13624..13848 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKQ94_RS15045 9419..10315 + 897 WP_002326827.1 ParA family protein -
KKQ94_RS15050 10407..10622 + 216 WP_001835296.1 peptide-binding protein -
KKQ94_RS15055 10640..10912 + 273 WP_002326825.1 antitoxin -
KKQ94_RS15060 10914..11777 + 864 WP_002332783.1 zeta toxin family protein -
KKQ94_RS15065 12340..12480 + 141 Protein_13 IS6 family transposase -
KKQ94_RS15070 12555..13235 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
KKQ94_RS15075 13290..13481 + 192 Protein_15 recombinase family protein -
KKQ94_RS15080 13624..13848 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
KKQ94_RS15085 13863..14732 + 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
KKQ94_RS15090 14713..15447 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
KKQ94_RS15095 15480..16388 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
KKQ94_RS15100 16385..16865 + 481 Protein_20 GNAT family N-acetyltransferase -
KKQ94_RS15105 16954..17634 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
KKQ94_RS15110 17843..18055 + 213 WP_002349227.1 hypothetical protein -
KKQ94_RS15115 18217..18735 + 519 WP_000357244.1 YfbU family protein -
KKQ94_RS15120 18742..19254 + 513 WP_000774078.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid cat(pC221) / erm(B) / ant(6)-Ia / VanHAX - 1..47525 47525
- inside IScluster/Tn cat(pC221) / erm(B) / ant(6)-Ia - 1345..22020 20675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T294696 WP_000233000.1 NZ_OU015948:13863-14732 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT294696 WP_000205227.1 NZ_OU015948:13624-13848 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References