Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 10640..11777 Replicon plasmid pl2
Accession NZ_OU015948
Organism Enterococcus faecium isolate USZ_VRE5_P5

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag KKQ94_RS15060 Protein ID WP_002332783.1
Coordinates 10914..11777 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag KKQ94_RS15055 Protein ID WP_002326825.1
Coordinates 10640..10912 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKQ94_RS15030 6730..7467 + 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
KKQ94_RS15035 7637..7897 + 261 Protein_7 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
KKQ94_RS15040 7968..9307 + 1340 WP_086953915.1 IS3-like element ISEnfa3 family transposase -
KKQ94_RS15045 9419..10315 + 897 WP_002326827.1 ParA family protein -
KKQ94_RS15050 10407..10622 + 216 WP_001835296.1 peptide-binding protein -
KKQ94_RS15055 10640..10912 + 273 WP_002326825.1 antitoxin Antitoxin
KKQ94_RS15060 10914..11777 + 864 WP_002332783.1 zeta toxin family protein Toxin
KKQ94_RS15065 12340..12480 + 141 Protein_13 IS6 family transposase -
KKQ94_RS15070 12555..13235 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
KKQ94_RS15075 13290..13481 + 192 Protein_15 recombinase family protein -
KKQ94_RS15080 13624..13848 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator -
KKQ94_RS15085 13863..14732 + 870 WP_000233000.1 nucleotidyltransferase domain-containing protein -
KKQ94_RS15090 14713..15447 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
KKQ94_RS15095 15480..16388 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid cat(pC221) / erm(B) / ant(6)-Ia / VanHAX - 1..47525 47525
- inside IScluster/Tn cat(pC221) / erm(B) / ant(6)-Ia - 1345..22020 20675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T294695 WP_002332783.1 NZ_OU015948:10914-11777 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT294695 WP_002326825.1 NZ_OU015948:10640-10912 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References