Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 18665..19773 Replicon plasmid pl2
Accession NZ_OU015711
Organism Enterococcus faecium isolate USZ_VRE32_P32

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag KKQ93_RS15915 Protein ID WP_000233000.1
Coordinates 18665..19534 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag KKQ93_RS15920 Protein ID WP_000205227.1
Coordinates 19549..19773 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKQ93_RS15880 14143..14655 - 513 WP_000774078.1 hypothetical protein -
KKQ93_RS15885 14662..15180 - 519 WP_000357244.1 YfbU family protein -
KKQ93_RS15890 15342..15554 - 213 WP_002349227.1 hypothetical protein -
KKQ93_RS15895 15763..16443 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
KKQ93_RS15900 16532..17012 - 481 Protein_20 GNAT family N-acetyltransferase -
KKQ93_RS15905 17009..17917 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
KKQ93_RS15910 17950..18684 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
KKQ93_RS15915 18665..19534 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
KKQ93_RS15920 19549..19773 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
KKQ93_RS15925 19916..20107 - 192 Protein_25 recombinase family protein -
KKQ93_RS15930 20162..20842 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
KKQ93_RS15935 20917..21057 - 141 Protein_27 IS6 family transposase -
KKQ93_RS15940 21620..22483 - 864 WP_002332783.1 zeta toxin family protein -
KKQ93_RS15945 22485..22757 - 273 WP_002326825.1 antitoxin -
KKQ93_RS15950 22775..22990 - 216 WP_001835296.1 peptide-binding protein -
KKQ93_RS15955 23082..23978 - 897 WP_002326827.1 ParA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ant(6)-Ia / erm(B) / cat(pC221) / VanHAX - 1..51289 51289
- inside IScluster/Tn ant(6)-Ia / erm(B) / cat(pC221) - 11377..32052 20675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T294689 WP_000233000.1 NZ_OU015711:c19534-18665 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT294689 WP_000205227.1 NZ_OU015711:c19773-19549 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References