Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 10473..11581 Replicon plasmid P03
Accession NZ_OU015353
Organism Enterococcus faecium strain AUS2002 isolate AUS2002

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag K3T69_RS15860 Protein ID WP_002303392.1
Coordinates 10473..11342 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag K3T69_RS15865 Protein ID WP_002303393.1
Coordinates 11357..11581 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3T69_RS15830 5769..6020 - 252 Protein_9 GNAT family N-acetyltransferase -
K3T69_RS15835 6265..7560 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
K3T69_RS15840 7661..8569 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
K3T69_RS15845 8593..8820 - 228 Protein_12 hypothetical protein -
K3T69_RS15850 8817..9725 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
K3T69_RS15855 9758..10492 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
K3T69_RS15860 10473..11342 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
K3T69_RS15865 11357..11581 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
K3T69_RS15870 11724..12545 - 822 Protein_17 recombinase family protein -
K3T69_RS15875 13233..14036 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
K3T69_RS15880 14090..15574 - 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1..37193 37193
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 672..36037 35365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T294684 WP_002303392.1 NZ_OU015353:c11342-10473 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT294684 WP_002303393.1 NZ_OU015353:c11581-11357 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References