Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1995..3132 Replicon plasmid P03
Accession NZ_OU015353
Organism Enterococcus faecium strain AUS2002 isolate AUS2002

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag K3T69_RS15805 Protein ID WP_001284311.1
Coordinates 2269..3132 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag K3T69_RS15800 Protein ID WP_000301765.1
Coordinates 1995..2267 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3T69_RS15780 1..603 + 603 Protein_0 replication initiation factor domain-containing protein -
K3T69_RS15785 678..1358 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
K3T69_RS15790 1414..1671 + 258 Protein_2 chromosome partitioning protein ParA -
K3T69_RS15795 1763..1978 + 216 WP_001835296.1 peptide-binding protein -
K3T69_RS15800 1995..2267 + 273 WP_000301765.1 antitoxin Antitoxin
K3T69_RS15805 2269..3132 + 864 WP_001284311.1 zeta toxin family protein Toxin
K3T69_RS15810 3395..4132 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
K3T69_RS15815 4257..4340 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
K3T69_RS15825 4882..5676 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
K3T69_RS15830 5769..6020 - 252 Protein_9 GNAT family N-acetyltransferase -
K3T69_RS15835 6265..7560 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1..37193 37193
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 672..36037 35365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T294683 WP_001284311.1 NZ_OU015353:2269-3132 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT294683 WP_000301765.1 NZ_OU015353:1995-2267 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References