Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 469781..470352 Replicon chromosome
Accession NZ_OU015350
Organism Enterococcus faecium strain AUS2002 isolate AUS2002

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag K3T69_RS02235 Protein ID WP_002286801.1
Coordinates 470011..470352 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag K3T69_RS02230 Protein ID WP_002323011.1
Coordinates 469781..470011 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3T69_RS02200 464915..466246 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
K3T69_RS02205 466268..466894 + 627 WP_002286816.1 cysteine hydrolase -
K3T69_RS02210 467077..467658 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
K3T69_RS02215 468390..468736 + 347 Protein_437 SOS response-associated peptidase -
K3T69_RS02220 468712..468951 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
K3T69_RS02225 469156..469494 - 339 WP_002286804.1 hypothetical protein -
K3T69_RS02230 469781..470011 + 231 WP_002323011.1 hypothetical protein Antitoxin
K3T69_RS02235 470011..470352 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K3T69_RS02240 471202..471387 + 186 WP_002304207.1 hypothetical protein -
K3T69_RS02245 471892..472086 + 195 WP_002297028.1 hypothetical protein -
K3T69_RS02250 472277..472526 + 250 Protein_444 transposase -
K3T69_RS02255 472770..473600 - 831 WP_002286789.1 manganese catalase family protein -
K3T69_RS02260 473808..475142 + 1335 WP_002303373.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T294680 WP_002286801.1 NZ_OU015350:470011-470352 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T294680 NZ_OU015350:470011-470352 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT294680 WP_002323011.1 NZ_OU015350:469781-470011 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT294680 NZ_OU015350:469781-470011 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References