Detailed information of TA system TA029460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1013253..1013872
• Replicon: chromosome
• Accession: NC_021251
• Organism: Mycobacterium tuberculosis CCDC5079

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CFBS_RS04795
• Protein ID: WP_003404726.1
• Coordinates: 1013438..1013872 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: CFBS_RS04790
• Protein ID: WP_003898641.1
• Coordinates: 1013253..1013432 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBS_RS04780	1008726..1010306	+	1581	WP_003910977.1	serine hydrolase	-
CFBS_RS04785	1010303..1012696	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
CFBS_RS22720	1012969..1013127	+	159	WP_003404720.1	hypothetical protein	-
CFBS_RS04790	1013253..1013432	+	180	WP_003898641.1	antitoxin	Antitoxin
CFBS_RS04795	1013438..1013872	+	435	WP_003404726.1	SRPBCC family protein	Toxin
CFBS_RS04800	1013970..1014743	+	774	WP_003404735.1	VOC family protein	-
CFBS_RS04805	1014808..1015257	+	450	WP_003404738.1	hypothetical protein	-
CFBS_RS04810	1015392..1015622	-	231	WP_003898642.1	hypothetical protein	-
CFBS_RS04815	1015789..1017297	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
CFBS_RS04820	1017299..1018537	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T29460 WP_003404726.1 NC_021251:1013438-1013872 [Mycobacterium tuberculosis CCDC5079]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T29460 NC_021251:1013438-1013872 [Mycobacterium tuberculosis CCDC5079]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT29460 WP_003898641.1 NC_021251:1013253-1013432 [Mycobacterium tuberculosis CCDC5079]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT29460 NC_021251:1013253-1013432 [Mycobacterium tuberculosis CCDC5079]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5