294589

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 580227..581335 Replicon chromosome
Accession NZ_OD940440
Organism Enterococcus faecalis isolate WE0254

Toxin (Protein)


Gene name MNTss Uniprot ID A0A2H4HI44
Locus tag KG893_RS03000 Protein ID WP_029170889.1
Coordinates 580466..581335 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag KG893_RS02995 Protein ID WP_000205227.1
Coordinates 580227..580451 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG893_RS02960 (575543) 575543..576223 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
KG893_RS02965 (576291) 576291..576618 + 328 Protein_569 recombinase family protein -
KG893_RS02970 (576618) 576618..577295 + 678 Protein_570 DNA topoisomerase -
KG893_RS14850 (577606) 577606..578103 + 498 WP_002327635.1 DNA recombinase -
KG893_RS02985 (578104) 578104..578520 + 417 WP_000323438.1 recombinase -
KG893_RS02990 (578540) 578540..580084 + 1545 WP_002390960.1 recombinase family protein -
KG893_RS02995 (580227) 580227..580451 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
KG893_RS03000 (580466) 580466..581335 + 870 WP_029170889.1 nucleotidyltransferase domain-containing protein Toxin
KG893_RS03005 (581316) 581316..582050 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
KG893_RS03010 (582083) 582083..582991 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
KG893_RS03015 (582988) 582988..583468 + 481 Protein_578 GNAT family N-acetyltransferase -
KG893_RS03020 (583561) 583561..584355 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
KG893_RS03030 (584897) 584897..584980 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
KG893_RS03035 (585105) 585105..585842 + 738 WP_001038790.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 575543..576223 680
- flank IS/Tn ant(6)-Ia / aph(3')-III / erm(B) - 582083..590605 8522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-114)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32820.25 Da        Isoelectric Point: 4.9564

>T294589 WP_029170889.1 NZ_OD940440:580466-581335 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT294589 WP_000205227.1 NZ_OD940440:580227-580451 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H4HI44

Antitoxin

Source ID Structure

References