Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1166673..1167244 Replicon chromosome
Accession NZ_OD940422
Organism Enterococcus faecalis isolate WE0851

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag KJA80_RS05450 Protein ID WP_002360937.1
Coordinates 1166673..1167014 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag KJA80_RS05455 Protein ID WP_002354773.1
Coordinates 1167014..1167244 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJA80_RS05445 (1162688) 1162688..1166302 - 3615 WP_010712913.1 DNA-directed RNA polymerase subunit beta -
KJA80_RS05450 (1166673) 1166673..1167014 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KJA80_RS05455 (1167014) 1167014..1167244 - 231 WP_002354773.1 hypothetical protein Antitoxin
KJA80_RS05460 (1167418) 1167418..1167633 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
KJA80_RS05465 (1167772) 1167772..1168764 + 993 WP_002358973.1 biotin--[acetyl-CoA-carboxylase] ligase -
KJA80_RS05470 (1169028) 1169028..1169666 - 639 WP_002375448.1 lytic polysaccharide monooxygenase -
KJA80_RS05475 (1170353) 1170353..1171969 + 1617 WP_002415597.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T294524 WP_002360937.1 NZ_OD940422:c1167014-1166673 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT294524 WP_002354773.1 NZ_OD940422:c1167244-1167014 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References