Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 36945..37516 Replicon plasmid contig000002
Accession NZ_OD940421
Organism Enterococcus faecalis isolate WE0438

Toxin (Protein)


Gene name mazF Uniprot ID A0A2Z2CGM4
Locus tag KJA91_RS14380 Protein ID WP_002362432.1
Coordinates 36945..37286 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag KJA91_RS14385 Protein ID WP_002362431.1
Coordinates 37286..37516 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJA91_RS14360 (WE0438_02941) 32446..33294 + 849 WP_001258486.1 streptomycin adenylyltransferase Str -
KJA91_RS14370 (WE0438_02944) 35028..35630 - 603 WP_002362434.1 Fic family protein -
KJA91_RS14375 (WE0438_02945) 35895..36833 - 939 WP_002362433.1 hypothetical protein -
KJA91_RS14380 (WE0438_02946) 36945..37286 - 342 WP_002362432.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KJA91_RS14385 (WE0438_02947) 37286..37516 - 231 WP_002362431.1 hypothetical protein Antitoxin
KJA91_RS14390 (WE0438_02948) 37720..38340 + 621 WP_013438829.1 recombinase family protein -
KJA91_RS14395 (WE0438_02949) 38357..38641 + 285 WP_002394798.1 hypothetical protein -
KJA91_RS14400 (WE0438_02950) 38643..38876 + 234 WP_002394799.1 hypothetical protein -
KJA91_RS14405 (WE0438_02951) 39036..39290 - 255 WP_002394800.1 hypothetical protein -
KJA91_RS14410 (WE0438_02952) 39407..40075 - 669 WP_002394801.1 CPBP family intramembrane metalloprotease -
KJA91_RS14415 (WE0438_02953) 40111..40428 - 318 WP_002394802.1 heavy metal-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(L) / tet(M) / str / optrA / fexA / erm(B) - 1..61284 61284


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13244.44 Da        Isoelectric Point: 8.0113

>T294514 WP_002362432.1 NZ_OD940421:c37286-36945 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT294514 WP_002362431.1 NZ_OD940421:c37516-37286 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z2CGM4

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References