Detailed information of TA system TA294490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2794089..2794716
• Replicon: chromosome
• Accession: NZ_NQJO01000001
• Organism: Erwinia amylovora strain CTBT3-1 tig00000000

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: CS942_RS13230
• Protein ID: WP_004156337.1
• Coordinates: 2794498..2794716 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CS942_RS13225
• Protein ID: WP_013035913.1
• Coordinates: 2794089..2794478 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS942_RS13195	2789108..2792254	+	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit AcrB	-
CS942_RS13200	2792402..2792671	+	270	WP_099258260.1	type B 50S ribosomal protein L31	-
CS942_RS13205	2792671..2792817	+	147	WP_004156349.1	type B 50S ribosomal protein L36	-
CS942_RS13220	2793589..2793942	+	354	WP_004156342.1	hypothetical protein	-
CS942_RS13225	2794089..2794478	+	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
CS942_RS13230	2794498..2794716	+	219	WP_004156337.1	HHA domain-containing protein	Toxin
CS942_RS13240	2795016..2795333	+	318	WP_004156334.1	MGMT family protein	-
CS942_RS13245	2795356..2795913	-	558	WP_004156333.1	YbaY family lipoprotein	-
CS942_RS13250	2796138..2796998	+	861	WP_004156324.1	acyl-CoA thioesterase II	-
CS942_RS13255	2797073..2798374	-	1302	WP_004172214.1	ammonium transporter AmtB	-
CS942_RS13260	2798392..2798730	-	339	WP_004156319.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T294490 WP_004156337.1 NZ_NQJO01000001:2794498-2794716 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T294490 NZ_NQJO01000001:2794498-2794716 [Erwinia amylovora]
ATGACTGACAAACTTCTAACGAAAACTGACTACCTGATGCGCCTCAGGCGTTGTCGTTCAATCGATACACTTGAACGCGT
GATTGAAAAGAACAAGTACGAATTATCTGATGATGAACTGGCTGTATTTTATTCAGCCGCCGATCACCGTCTTGCTGAAC
TAACCATGAATAAGCTGTACGACAAAGTACCGGTCGCTGTCTGGAAGTTTGTCCGTTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT294490 WP_013035913.1 NZ_NQJO01000001:2794089-2794478 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT294490 NZ_NQJO01000001:2794089-2794478 [Erwinia amylovora]
ATGGACGAATACTCACCGAAAAGGCATGATATTGCGCAGCTGAAATTCTTATGTGAAAACCTGTATGATGAAAGTCTGGC
TACGTTAGGCGACAGCCATCATGGCTGGGTTAACGACCCCACCTCAACGAGCAATCTTCAGCTTAACGACCTGATAGAGC
ACATTGCAGCATTCACAATGAATTACAAAATCAAGCATATCGAAGACAGCGATCTGATAAGTCAAATTGATGAGTATCTG
GACGACACCTTTATGCTTTTCAGCAATTACGGTGTCAACAATCTTGACTTACAACGCTGGCAAAAATCAGCGAAGCGATT
GTTTAACATTTTTGCTAAAGAATGCGTAATGAGCCAGATCCAGTCCAGCCATTCATTTAGCAGCCCATAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0