Detailed information of TA system TA294404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2395622..2397344
• Replicon: chromosome
• Accession: NZ_LT992492
• Organism: Vibrio cholerae strain 4295STDY6534248

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: D0FZT9
• Locus tag: DG169_RS11225
• Protein ID: WP_000356587.1
• Coordinates: 2395622..2396560 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A934MVJ4
• Locus tag: DG169_RS11230
• Protein ID: WP_000095577.1
• Coordinates: 2396553..2397344 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DG169_RS11205	2391563..2392075	-	513	WP_001166556.1	S26 family signal peptidase	-
DG169_RS11210	2392059..2392406	-	348	WP_001044650.1	hypothetical protein	-
DG169_RS11215	2392399..2394798	-	2400	WP_000648041.1	type IV secretion system protein TraC	-
DG169_RS11220	2394798..2395490	-	693	WP_001228924.1	DsbC family protein	-
DG169_RS11225	2395622..2396560	-	939	WP_000356587.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
DG169_RS11230	2396553..2397344	-	792	WP_000095577.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
DG169_RS11235	2397565..2397951	-	387	WP_000180225.1	hypothetical protein	-
DG169_RS11240	2397948..2398520	-	573	WP_139057167.1	type IV conjugative transfer system lipoprotein TraV	-
DG169_RS11245	2398595..2399884	-	1290	WP_001883765.1	TraB/VirB10 family protein	-
DG169_RS11250	2399887..2400783	-	897	WP_032467354.1	type-F conjugative transfer system secretin TraK	-
DG169_RS11255	2400767..2401393	-	627	WP_000667169.1	hypothetical protein	-
DG169_RS11260	2401390..2401671	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
DG169_RS11265	2401852..2402175	+	324	WP_000118545.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2310264..2443147	132883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35548.82 Da
• Isoelectric Point: 4.9081

>T294404 WP_000356587.1 NZ_LT992492:c2396560-2395622 [Vibrio cholerae]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30109.49 Da
• Isoelectric Point: 9.5097

>AT294404 WP_000095577.1 NZ_LT992492:c2397344-2396553 [Vibrio cholerae]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

Structures

Toxin

• AlphaFold DB: A0A1U9VZ44