Detailed information of TA system TA294403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2184682..2186507
• Replicon: chromosome
• Accession: NZ_LT992492
• Organism: Vibrio cholerae strain 4295STDY6534248

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: DG169_RS10295
• Protein ID: WP_000392003.1
• Coordinates: 2184682..2186043 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: DG169_RS10300
• Protein ID: WP_000804849.1
• Coordinates: 2186040..2186507 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DG169_RS10275	2180533..2182053	+	1521	WP_000640055.1	aldehyde dehydrogenase	-
DG169_RS10280	2182395..2183075	+	681	Protein_1920	IS5 family transposase	-
DG169_RS10285	2183101..2184078	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
DG169_RS10295	2184682..2186043	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
DG169_RS10300	2186040..2186507	+	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
DG169_RS10305	2186935..2187513	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
DG169_RS10310	2187517..2190453	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
DG169_RS10315	2190523..2191227	+	705	WP_001880605.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	acfA / acfC / acfB / tcpJ / tcpN/toxT / tcpF / tcpE / tcpT / tcpS / tcpD / tcpR / tcpC / tcpQ / tcpB / tcpA / tcpH / tcpP / tcpI	2151120..2192070	40950
	-	flank	IS/Tn	-	-	2183101..2183853	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T294403 WP_000392003.1 NZ_LT992492:2184682-2186043 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT294403 WP_000804849.1 NZ_LT992492:2186040-2186507 [Vibrio cholerae]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8