Detailed information of TA system TA294385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2190673..2192498
• Replicon: chromosome
• Accession: NZ_LT992490
• Organism: Vibrio cholerae strain 4295STDY6534200

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HUW69_RS09790
• Protein ID: WP_000391997.1
• Coordinates: 2190673..2192034 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUW69_RS09795
• Protein ID: WP_002020638.1
• Coordinates: 2192031..2192498 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW69_RS09775	2186181..2186834	-	654	WP_148524106.1	inovirus Gp2 family protein	-
HUW69_RS09780	2187204..2188199	+	996	WP_148524107.1	hypothetical protein	-
HUW69_RS09785	2188708..2190078	-	1371	WP_001927459.1	Fic family protein	-
HUW69_RS09790	2190673..2192034	+	1362	WP_000391997.1	type II toxin-antitoxin system HipA family toxin	Toxin
HUW69_RS09795	2192031..2192498	+	468	WP_002020638.1	helix-turn-helix domain-containing protein	Antitoxin
HUW69_RS09800	2192926..2193504	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
HUW69_RS09805	2193508..2196456	+	2949	WP_113606009.1	DEAD/DEAH box helicase family protein	-
HUW69_RS09810	2196527..2197228	+	702	Protein_1900	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2170527..2199842	29315
	-	flank	IS/Tn	-	-	2197292..2198902	1610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51307.21 Da
• Isoelectric Point: 4.9109

>T294385 WP_000391997.1 NZ_LT992490:2190673-2192034 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFFFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17077.54 Da
• Isoelectric Point: 10.7084

>AT294385 WP_002020638.1 NZ_LT992490:2192031-2192498 [Vibrio cholerae]
MKPSNKESVQDTVARLRANRNQQQPKVGKNATHSSKEQTPLDTPTRSKASKNAKTVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp