Detailed information of TA system TA294366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2395623..2397345
• Replicon: chromosome
• Accession: NZ_LT992488
• Organism: Vibrio cholerae strain 4295STDY6534232

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: D0FZT9
• Locus tag: DG176_RS11225
• Protein ID: WP_000356587.1
• Coordinates: 2395623..2396561 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A934MVJ4
• Locus tag: DG176_RS11230
• Protein ID: WP_000095577.1
• Coordinates: 2396554..2397345 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DG176_RS11205	2391564..2392076	-	513	WP_001166556.1	S26 family signal peptidase	-
DG176_RS11210	2392060..2392407	-	348	WP_001044650.1	hypothetical protein	-
DG176_RS11215	2392400..2394799	-	2400	WP_000648041.1	type IV secretion system protein TraC	-
DG176_RS11220	2394799..2395491	-	693	WP_001228924.1	DsbC family protein	-
DG176_RS11225	2395623..2396561	-	939	WP_000356587.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
DG176_RS11230	2396554..2397345	-	792	WP_000095577.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
DG176_RS11235	2397566..2397952	-	387	WP_000180225.1	hypothetical protein	-
DG176_RS11240	2397949..2398521	-	573	WP_139057167.1	type IV conjugative transfer system lipoprotein TraV	-
DG176_RS11245	2398596..2399885	-	1290	WP_001883765.1	TraB/VirB10 family protein	-
DG176_RS11250	2399888..2400784	-	897	WP_032467354.1	type-F conjugative transfer system secretin TraK	-
DG176_RS11255	2400768..2401394	-	627	WP_000667169.1	hypothetical protein	-
DG176_RS11260	2401391..2401672	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
DG176_RS11265	2401853..2402176	+	324	WP_000118545.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2310265..2443148	132883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35548.82 Da
• Isoelectric Point: 4.9081

>T294366 WP_000356587.1 NZ_LT992488:c2396561-2395623 [Vibrio cholerae]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30109.49 Da
• Isoelectric Point: 9.5097

>AT294366 WP_000095577.1 NZ_LT992488:c2397345-2396554 [Vibrio cholerae]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

Structures

Toxin

• AlphaFold DB: A0A1U9VZ44