Detailed information of TA system TA294365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2184683..2186508
• Replicon: chromosome
• Accession: NZ_LT992488
• Organism: Vibrio cholerae strain 4295STDY6534232

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: DG176_RS10295
• Protein ID: WP_000392003.1
• Coordinates: 2184683..2186044 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: DG176_RS10300
• Protein ID: WP_000804849.1
• Coordinates: 2186041..2186508 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DG176_RS10275	2180534..2182054	+	1521	WP_000640055.1	aldehyde dehydrogenase	-
DG176_RS10280	2182396..2183076	+	681	Protein_1920	IS5 family transposase	-
DG176_RS10285	2183102..2184079	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
DG176_RS10295	2184683..2186044	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
DG176_RS10300	2186041..2186508	+	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
DG176_RS10305	2186936..2187514	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
DG176_RS10310	2187518..2190454	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
DG176_RS10315	2190524..2191228	+	705	WP_001880605.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	acfA / acfC / acfB / tcpJ / tcpN/toxT / tcpF / tcpE / tcpT / tcpS / tcpD / tcpR / tcpC / tcpQ / tcpB / tcpA / tcpH / tcpP / tcpI	2151121..2192071	40950
	-	flank	IS/Tn	-	-	2183102..2183854	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T294365 WP_000392003.1 NZ_LT992488:2184683-2186044 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT294365 WP_000804849.1 NZ_LT992488:2186041-2186508 [Vibrio cholerae]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8