Detailed information of TA system TA029424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3343355..3344008
• Replicon: chromosome
• Accession: NC_021200
• Organism: Mycobacterium avium subsp. paratuberculosis MAP4

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MAP4_RS15005
• Protein ID: WP_003872880.1
• Coordinates: 3343355..3343816 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: MAP4_RS15010
• Protein ID: WP_003872881.1
• Coordinates: 3343820..3344008 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAP4_RS14990	3339847..3340233	-	387	WP_003872877.1	hypothetical protein	-
MAP4_RS14995	3340273..3341856	-	1584	WP_003872878.1	DUF4185 domain-containing protein	-
MAP4_RS15000	3342852..3343286	-	435	WP_003877359.1	hypothetical protein	-
MAP4_RS15005	3343355..3343816	-	462	WP_003872880.1	SRPBCC family protein	Toxin
MAP4_RS15010	3343820..3344008	-	189	WP_003872881.1	antitoxin	Antitoxin
MAP4_RS15015	3344071..3346443	-	2373	WP_003872882.1	HAD-IC family P-type ATPase	-
MAP4_RS15020	3346440..3348026	-	1587	WP_003872883.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16626.19 Da
• Isoelectric Point: 9.5654

>T29424 WP_003872880.1 NC_021200:c3343816-3343355 [Mycobacterium avium subsp. paratuberculosis MAP4]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQIDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T29424 NC_021200:c3343816-3343355 [Mycobacterium avium subsp. paratuberculosis MAP4]
GTGGCCAAGCTTTCCGGATCCATCGATGTTCCGCTGCCCCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGATCGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAAGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGGATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGTTCCGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCCATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT29424 WP_003872881.1 NC_021200:c3344008-3343820 [Mycobacterium avium subsp. paratuberculosis MAP4]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT29424 NC_021200:c3344008-3343820 [Mycobacterium avium subsp. paratuberculosis MAP4]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATGGCCATCGACAAGGCCGGCGA
GTTCGTCGACGAGAAGACCCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCG
CCACCGAGACCGGCGACCAGCAGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18