Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1194842..1195406 Replicon chromosome
Accession NC_021200
Organism Mycobacterium avium subsp. paratuberculosis MAP4

Toxin (Protein)


Gene name vapC Uniprot ID X7VBD8
Locus tag MAP4_RS05665 Protein ID WP_003875391.1
Coordinates 1195035..1195406 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7L5MGM2
Locus tag MAP4_RS22590 Protein ID WP_003875390.1
Coordinates 1194842..1195048 (+) Length 69 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAP4_RS05645 1190095..1191093 + 999 WP_003875386.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
MAP4_RS05650 1191117..1192325 - 1209 WP_010949696.1 hypothetical protein -
MAP4_RS05655 1192456..1193529 + 1074 WP_003878533.1 redox-regulated ATPase YchF -
MAP4_RS05660 1193729..1194673 + 945 WP_003875389.1 HNH endonuclease -
MAP4_RS22590 1194842..1195048 + 207 WP_003875390.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MAP4_RS05665 1195035..1195406 + 372 WP_003875391.1 type II toxin-antitoxin system VapC family toxin Toxin
MAP4_RS05670 1195506..1195946 + 441 WP_010949694.1 nitroreductase family deazaflavin-dependent oxidoreductase -
MAP4_RS05675 1196002..1196277 + 276 WP_003878531.1 hypothetical protein -
MAP4_RS05680 1196379..1196777 + 399 WP_003875394.1 VOC family protein -
MAP4_RS22595 1196844..1197227 + 384 WP_003875395.1 hypothetical protein -
MAP4_RS05685 1197297..1197650 + 354 WP_003875396.1 hypothetical protein -
MAP4_RS05690 1197807..1198331 + 525 WP_033713819.1 glycoside hydrolase -
MAP4_RS05695 1198742..1199066 + 325 Protein_1146 antibiotic biosynthesis monooxygenase -
MAP4_RS05700 1199071..1199754 - 684 WP_003875399.1 hypothetical protein -
MAP4_RS05705 1199872..1200069 + 198 WP_016706054.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13770.14 Da        Isoelectric Point: 6.6003

>T29421 WP_003875391.1 NC_021200:1195035-1195406 [Mycobacterium avium subsp. paratuberculosis MAP4]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Download         Length: 372 bp

>T29421 NC_021200:1195035-1195406 [Mycobacterium avium subsp. paratuberculosis MAP4]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin


Download         Length: 69 a.a.        Molecular weight: 7231.22 Da        Isoelectric Point: 11.0634

>AT29421 WP_003875390.1 NC_021200:1194842-1195048 [Mycobacterium avium subsp. paratuberculosis MAP4]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Download         Length: 207 bp

>AT29421 NC_021200:1194842-1195048 [Mycobacterium avium subsp. paratuberculosis MAP4]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2WB37

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MGM2

References