Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 93050..94673 Replicon plasmid pC45-VIM4
Accession NZ_LT991958
Organism Enterobacter cloacae complex sp. isolate C45

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag DBR02_RS24005 Protein ID WP_000088044.1
Coordinates 93348..94673 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag DBR02_RS24000 Protein ID WP_012477377.1
Coordinates 93050..93343 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR02_RS23975 88849..89241 + 393 WP_000341066.1 hypothetical protein -
DBR02_RS23985 89900..90556 + 657 WP_000462754.1 trypsin-like serine protease -
DBR02_RS23990 90759..91256 + 498 WP_000340139.1 membrane protein -
DBR02_RS23995 91261..92649 + 1389 WP_012695445.1 hypothetical protein -
DBR02_RS24000 93050..93343 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
DBR02_RS24005 93348..94673 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
DBR02_RS24010 94734..94940 + 207 WP_000134171.1 hypothetical protein -
DBR02_RS24015 95041..95451 + 411 WP_000985909.1 hypothetical protein -
DBR02_RS24020 95464..95892 + 429 Protein_91 hypothetical protein -
DBR02_RS24025 96170..97174 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaVIM-4 / aac(6')-Il / dfrA1 / aadA1 / qacE / sul1 / blaTEM-1B / ant(2'')-Ia / aadA2 / qnrA1 / tet(A) / mcr-9 - 1..299117 299117


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T294004 WP_000088044.1 NZ_LT991958:93348-94673 [Enterobacter cloacae complex sp.]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT294004 WP_012477377.1 NZ_LT991958:93050-93343 [Enterobacter cloacae complex sp.]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References