Detailed information of TA system TA293990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 178890..180513
• Replicon: plasmid pC309-p2
• Accession: NZ_LT991956
• Organism: Enterobacter hormaechei subsp. steigerwaltii isolate C309

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: DBR08_RS25605
• Protein ID: WP_047343497.1
• Coordinates: 179188..180513 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: DBR08_RS25600
• Protein ID: WP_032636197.1
• Coordinates: 178890..179183 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR08_RS25575	174251..174706	+	456	WP_045342027.1	hypothetical protein	-
DBR08_RS25580	174886..175398	+	513	WP_023327709.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
DBR08_RS25585	175740..176396	+	657	WP_045342030.1	trypsin-like serine protease	-
DBR08_RS25590	176599..177096	+	498	WP_045342032.1	membrane protein	-
DBR08_RS25595	177101..178489	+	1389	WP_058672718.1	hypothetical protein	-
DBR08_RS25600	178890..179183	+	294	WP_032636197.1	helix-turn-helix transcriptional regulator	Antitoxin
DBR08_RS25605	179188..180513	+	1326	WP_047343497.1	type II toxin-antitoxin system HipA family toxin	Toxin
DBR08_RS25610	180716..181126	+	411	WP_023327703.1	hypothetical protein	-
DBR08_RS25615	181139..181675	+	537	Protein_206	HNH endonuclease	-
DBR08_RS25620	181948..182433	-	486	WP_013087269.1	GNAT family N-acetyltransferase	-
DBR08_RS25625	182421..182687	-	267	WP_007869691.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..183339	183339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50206.19 Da
• Isoelectric Point: 6.2897

>T293990 WP_047343497.1 NZ_LT991956:179188-180513 [Enterobacter hormaechei subsp. steigerwaltii]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKCHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAEHDKAQFFMTQIIFWMMAATDGHAKNFS
ISVGPQGRYHLTPLYDVLSAWPVIGQGNNQISLQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPYQFHQQLVESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10989.46 Da
• Isoelectric Point: 10.5348

>AT293990 WP_032636197.1 NZ_LT991956:178890-179183 [Enterobacter hormaechei subsp. steigerwaltii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTTSSERK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp