Detailed information of TA system TA029399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1534386..1535079
• Replicon: chromosome
• Accession: NC_021173
• Organism: Burkholderia thailandensis MSMB121

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A132EML3
• Locus tag: BTI_RS07025
• Protein ID: WP_015601891.1
• Coordinates: 1534702..1535079 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: BTI_RS07020
• Protein ID: WP_015600977.1
• Coordinates: 1534386..1534721 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTI_RS07005	1530502..1531050	+	549	WP_015600544.1	hypothetical protein	-
BTI_RS07010	1531043..1533406	+	2364	Protein_1405	DEAD/DEAH box helicase family protein	-
BTI_RS07015	1533516..1534196	+	681	WP_015601902.1	hypothetical protein	-
BTI_RS33165	1534234..1534389	-	156	Protein_1407	HigA family addiction module antidote protein	-
BTI_RS07020	1534386..1534721	-	336	WP_015600977.1	helix-turn-helix domain-containing protein	Antitoxin
BTI_RS07025	1534702..1535079	-	378	WP_015601891.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTI_RS07030	1535491..1536960	+	1470	WP_015601175.1	aldehyde dehydrogenase family protein	-
BTI_RS07035	1537097..1537618	-	522	WP_015602266.1	DUF2867 domain-containing protein	-
BTI_RS07040	1537865..1538494	-	630	WP_015602117.1	TetR/AcrR family transcriptional regulator	-
BTI_RS07045	1538940..1540070	+	1131	WP_015601089.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1528317..1540070	11753

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13718.79 Da
• Isoelectric Point: 8.8594

>T29399 WP_015601891.1 NC_021173:c1535079-1534702 [Burkholderia thailandensis MSMB121]
MADKEKPLEWIASSYKDLMALPPDVRRRFGYALSLAQMGDQDDAAKVLKGFGGAGVLEVVEDDTGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKGDMDIIRARLKVAEALAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T29399 NC_021173:c1535079-1534702 [Burkholderia thailandensis MSMB121]
ATGGCCGACAAAGAAAAACCTCTCGAATGGATTGCGAGCAGCTACAAGGATCTGATGGCGTTGCCGCCCGATGTTCGCCG
GCGCTTCGGTTACGCGCTGTCGTTAGCGCAGATGGGCGACCAGGACGATGCGGCAAAGGTGCTCAAGGGCTTCGGCGGAG
CCGGCGTGCTAGAGGTCGTCGAGGATGATACCGGCGGCACGTACCGGGCCGTCTATACAGTCAAGTTCGCCGAGGCGGTG
TTCGTCCTGCACTGCTTCCAGAAAAAGAGCAAGAGTGGGATCGCCACGCCGAAAGGCGACATGGACATCATTCGCGCTCG
GTTGAAAGTGGCCGAAGCATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12152.36 Da
• Isoelectric Point: 10.2130

>AT29399 WP_015600977.1 NC_021173:c1534721-1534386 [Burkholderia thailandensis MSMB121]
MQKRIIEGVEVQRGSGNVYADLGLPDAEKLKIKTGLVIEIRKAMRALGLTQQAAAKRMGIPQPKVSGMMHGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLAVA

Nucleotide

• Length: 336 bp

>AT29399 NC_021173:c1534721-1534386 [Burkholderia thailandensis MSMB121]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCGGTTCGGGCAATGTCTATGCCGACCTCGGCTTGCCCGATGC
AGAAAAACTGAAGATCAAGACCGGCTTGGTAATCGAAATCAGAAAAGCCATGCGTGCCCTCGGACTCACTCAACAAGCCG
CGGCGAAACGCATGGGCATTCCGCAGCCGAAGGTGTCCGGCATGATGCATGGTGACTTCACTAACCTGTCCGAGCGCAAG
CTGATGGACTGCTTGAATCGGCTAGGCTACGACATCGAGATCAAGGTACGGCCGGCCGCCGAACCGATCGGGCATCTCAC
GCTCGCGGTCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A132EML3