Detailed information of TA system TA293983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 93049..94672
• Replicon: plasmid pC309-VIM4
• Accession: NZ_LT991955
• Organism: Enterobacter hormaechei subsp. steigerwaltii isolate C309

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: DBR08_RS23555
• Protein ID: WP_107591266.1
• Coordinates: 93347..94672 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: DBR08_RS23550
• Protein ID: WP_012477377.1
• Coordinates: 93049..93342 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR08_RS23525	88848..89240	+	393	WP_000341066.1	hypothetical protein	-
DBR08_RS23535	89899..90555	+	657	WP_000462754.1	trypsin-like serine protease	-
DBR08_RS23540	90758..91255	+	498	WP_000340139.1	membrane protein	-
DBR08_RS23545	91260..92648	+	1389	WP_012695445.1	hypothetical protein	-
DBR08_RS23550	93049..93342	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
DBR08_RS23555	93347..94672	+	1326	WP_107591266.1	type II toxin-antitoxin system HipA family toxin	Toxin
DBR08_RS23560	94733..94939	+	207	WP_000134171.1	hypothetical protein	-
DBR08_RS23565	95040..95450	+	411	WP_000985909.1	hypothetical protein	-
DBR08_RS23570	95463..95987	+	525	WP_107591267.1	hypothetical protein	-
DBR08_RS23575	96169..97173	-	1005	WP_000427614.1	IS110-like element IS5075 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaVIM-4 / aac(6')-Il / dfrA1 / aadA1 / qacE / sul1	-	1..254277	254277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50246.24 Da
• Isoelectric Point: 6.5044

>T293983 WP_107591266.1 NZ_LT991955:93347-94672 [Enterobacter hormaechei subsp. steigerwaltii]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISLQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT293983 WP_012477377.1 NZ_LT991955:93049-93342 [Enterobacter hormaechei subsp. steigerwaltii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

Structures

Antitoxin

• AlphaFold DB: A0A6M3PBR7