Detailed information of TA system TA293980

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 4338852..4339578
• Replicon: chromosome
• Accession: NZ_LT991954
• Organism: Enterobacter hormaechei subsp. steigerwaltii isolate C309

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: DBR08_RS21035
• Protein ID: WP_072035667.1
• Coordinates: 4339249..4339578 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: DBR08_RS21030
• Protein ID: WP_042893201.1
• Coordinates: 4338852..4339211 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR08_RS20980	4334348..4334755	+	408	WP_042893215.1	hypothetical protein	-
DBR08_RS20985	4334828..4335055	+	228	WP_042893214.1	hypothetical protein	-
DBR08_RS20990	4335136..4335300	+	165	WP_154816236.1	hypothetical protein	-
DBR08_RS20995	4335358..4336194	+	837	WP_107591249.1	hypothetical protein	-
DBR08_RS21000	4336288..4336521	+	234	WP_042893208.1	DUF905 family protein	-
DBR08_RS21005	4336619..4337437	+	819	WP_042893207.1	DUF945 domain-containing protein	-
DBR08_RS21010	4337437..4337691	+	255	WP_072035668.1	hypothetical protein	-
DBR08_RS21015	4337707..4338102	+	396	WP_045341441.1	antirestriction protein	-
DBR08_RS21020	4338114..4338593	+	480	WP_042893205.1	DNA repair protein RadC	-
DBR08_RS21025	4338608..4338829	+	222	WP_042893203.1	DUF987 domain-containing protein	-
DBR08_RS21030	4338852..4339211	+	360	WP_042893201.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DBR08_RS21035	4339249..4339578	+	330	WP_072035667.1	TA system toxin CbtA family protein	Toxin
DBR08_RS21040	4340208..4341446	+	1239	WP_045341443.1	ribokinase	-
DBR08_RS21045	4341514..4342491	+	978	WP_017692843.1	sugar ABC transporter permease	-
DBR08_RS21050	4342491..4343390	+	900	WP_017692842.1	carbohydrate ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4307023..4339578	32555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (15-115)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12450.17 Da
• Isoelectric Point: 10.5232

>T293980 WP_072035667.1 NZ_LT991954:4339249-4339578 [Enterobacter hormaechei subsp. steigerwaltii]
MQTLSSHSTRATQPNLSPVKTWQRLLTHLLSQHYGLRLNDTPFSNETTIREHIDAGVSLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T293980 NZ_LT991954:4339249-4339578 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCAAACCTTATCATCACACTCGACACGGGCGACTCAGCCCAATCTGTCACCCGTTAAAACCTGGCAACGGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTGAGGTTGAATGACACACCGTTCAGTAATGAAACCACCATACGAGAACATATTG
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAGTACGGGCTTGTCCGTATCGATCGGAAGGGATTT
TCATGGCAAGAACAAACCCCGTATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13412.23 Da
• Isoelectric Point: 5.8752

>AT293980 WP_042893201.1 NZ_LT991954:4338852-4339211 [Enterobacter hormaechei subsp. steigerwaltii]
MQNTTPVANHDIAEPWWGLKPTVTPCLGARLVQEGNRLHYLADPASITGTFNNEDLRHLDQAFPVLLKQLELMLVSGELT
SRYQHRITLYHNRLTCEADTLGSCGYVCIAIYPDQPEPH

Nucleotide

• Length: 360 bp

>AT293980 NZ_LT991954:4338852-4339211 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCAGAACACAACACCTGTAGCAAACCATGATATTGCCGAACCCTGGTGGGGACTTAAACCCACCGTCACGCCGTGTCT
TGGCGCACGGCTGGTACAGGAAGGCAACCGGCTGCATTATCTGGCTGACCCGGCAAGTATCACCGGAACATTCAACAATG
AGGATTTACGTCATCTCGACCAGGCGTTTCCGGTATTGCTTAAACAGCTGGAGTTGATGCTCGTTTCCGGTGAGCTCACC
TCCCGCTATCAGCACCGCATCACGCTATATCACAATAGACTGACTTGCGAAGCTGACACACTCGGCTCCTGTGGCTATGT
CTGCATCGCTATTTATCCCGACCAGCCTGAACCCCATTAA