Detailed information of TA system TA029396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 210966..211602
• Replicon: chromosome
• Accession: NC_021171
• Organism: Bacillus sp. 1NLA3E

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: N0AML2
• Locus tag: B1NLA3E_RS01120
• Protein ID: WP_015592038.1
• Coordinates: 211252..211602 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: N0APH5
• Locus tag: B1NLA3E_RS01115
• Protein ID: WP_015592037.1
• Coordinates: 210966..211247 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1NLA3E_RS01095	206976..207710	-	735	WP_015592033.1	rhomboid family intramembrane serine protease	-
B1NLA3E_RS01100	207819..208184	+	366	WP_015592034.1	holo-ACP synthase	-
B1NLA3E_RS01105	208368..209381	+	1014	WP_015592035.1	outer membrane lipoprotein carrier protein LolA	-
B1NLA3E_RS01110	209641..210801	+	1161	WP_041580210.1	alanine racemase	-
B1NLA3E_RS01115	210966..211247	+	282	WP_015592037.1	hypothetical protein	Antitoxin
B1NLA3E_RS01120	211252..211602	+	351	WP_015592038.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B1NLA3E_RS01125	211882..214059	+	2178	WP_015592039.1	RNA-binding transcriptional accessory protein	-
B1NLA3E_RS24585	214078..214191	-	114	WP_144061411.1	cortex morphogenetic protein CmpA	-
B1NLA3E_RS01130	214286..214759	+	474	WP_015592040.1	SprT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13006.03 Da
• Isoelectric Point: 4.8781

>T29396 WP_015592038.1 NC_021171:211252-211602 [Bacillus sp. 1NLA3E]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDDALQVSLGLIEF

Nucleotide

• Length: 351 bp

>T29396 NC_021171:211252-211602 [Bacillus sp. 1NLA3E]
TTGATTGTCAAACGTGGTGACGTATATTTTGCGGACCTATCCCCAGTTGTTGGCTCAGAACAAGGCGGTGTTCGTCCTGT
GCTTATCATTCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTCATTATTGCAGCAATTACAGCTCAAATACAAAAAG
CAAAGCTGCCTACCCATGTTGAAATTGATGCAAAGCGTTACGGCTTTGAACGAGACTCGGTCATTCTTTTAGAACAAATT
CGAACAATTGATAAACAACGACTTACCGATAAAATTACCCATCTCGACGACGAAATGATGGATAAAGTGGATGACGCCTT
ACAAGTTAGTTTAGGGCTCATCGAATTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10751.27 Da
• Isoelectric Point: 7.2028

>AT29396 WP_015592037.1 NC_021171:210966-211247 [Bacillus sp. 1NLA3E]
VSESSATTEILVKLPQHLLIELDGFVKQENGNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHAVERLVSGG

Nucleotide

• Length: 282 bp

>AT29396 NC_021171:210966-211247 [Bacillus sp. 1NLA3E]
GTGTCTGAATCCAGCGCAACAACTGAGATTTTGGTAAAATTACCGCAACATCTTTTAATCGAGTTGGATGGTTTCGTAAA
ACAGGAAAACGGTAATCGTAGTGAATTTATTTATCAAGCAACGAAAATGTATCTTCGCGAACGAAAAAAGAGACAAATTC
GCGAATCTATGAGACGAGGCTACATGGAAATGGCCAAAATTAATCTGACAATTGCTTCGGAAGCATTCCAAGCAGAGTAT
GAGGCAGAACACGCAGTAGAACGCCTAGTAAGCGGAGGGTAA

Structures

Toxin

• AlphaFold DB: N0AML2

Antitoxin

• AlphaFold DB: N0APH5