Detailed information of TA system TA293949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5656901..5657487
• Replicon: chromosome
• Accession: NZ_LT969520
• Organism: Pseudomonas aeruginosa isolate RW109

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: RW109_RS30540
• Protein ID: WP_003120987.1
• Coordinates: 5657188..5657487 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: RW109_RS30535
• Protein ID: WP_003448662.1
• Coordinates: 5656901..5657191 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RW109_RS30515	5652052..5652261	+	210	WP_003105733.1	cold-shock protein	-
RW109_RS30520	5652483..5654372	+	1890	WP_016851610.1	hypothetical protein	-
RW109_RS30525	5654369..5656345	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
RW109_RS30530	5656486..5656830	+	345	WP_016851612.1	hypothetical protein	-
RW109_RS30535	5656901..5657191	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
RW109_RS30540	5657188..5657487	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RW109_RS30545	5657689..5658813	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
RW109_RS30550	5658813..5660522	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
RW109_RS30555	5660526..5661851	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
RW109_RS30560	5661841..5662374	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5632149..5657487	25338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T293949 WP_003120987.1 NZ_LT969520:c5657487-5657188 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT293949 WP_003448662.1 NZ_LT969520:c5657191-5656901 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

Structures

Toxin

• AlphaFold DB: G8CP73