Detailed information of TA system TA029392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4106568..4107176
• Replicon: chromosome
• Accession: NC_021150
• Organism: Azotobacter vinelandii CA6

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: M9YR94
• Locus tag: AVCA6_RS18490
• Protein ID: WP_012702568.1
• Coordinates: 4106886..4107176 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AVCA6_RS18485
• Protein ID: WP_012702567.1
• Coordinates: 4106568..4106876 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVCA6_RS25960	4101749..4102087	+	339	WP_144061665.1	hypothetical protein	-
AVCA6_RS25965	4102093..4102311	+	219	WP_144061666.1	hypothetical protein	-
AVCA6_RS18445	4102339..4103229	-	891	Protein_3703	IS3-like element ISAzvi9 family transposase	-
AVCA6_RS18455	4103303..4104168	+	866	Protein_3704	IS110 family transposase	-
AVCA6_RS18460	4104422..4104685	-	264	Protein_3705	transposase	-
AVCA6_RS18465	4104896..4105120	+	225	WP_012702563.1	AlpA family phage regulatory protein	-
AVCA6_RS18470	4105122..4105505	+	384	WP_012702564.1	hypothetical protein	-
AVCA6_RS18475	4105502..4106173	+	672	WP_012702565.1	Rha family transcriptional regulator	-
AVCA6_RS18480	4106170..4106418	+	249	WP_012702566.1	hypothetical protein	-
AVCA6_RS18485	4106568..4106876	-	309	WP_012702567.1	putative addiction module antidote protein	Antitoxin
AVCA6_RS18490	4106886..4107176	-	291	WP_012702568.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AVCA6_RS26315	4107531..4107704	+	174	WP_012702569.1	hypothetical protein	-
AVCA6_RS18495	4107701..4107955	+	255	WP_012702570.1	hypothetical protein	-
AVCA6_RS18500	4107952..4108251	+	300	WP_012702571.1	hypothetical protein	-
AVCA6_RS25970	4108299..4108664	+	366	WP_012702573.1	hypothetical protein	-
AVCA6_RS18510	4108743..4109129	+	387	WP_012702575.1	toprim domain-containing protein	-
AVCA6_RS18515	4109126..4109518	+	393	WP_012702576.1	hypothetical protein	-
AVCA6_RS18520	4110222..4110425	+	204	WP_012702577.1	hypothetical protein	-
AVCA6_RS18525	4110920..4111129	+	210	WP_012702578.1	hypothetical protein	-
AVCA6_RS18530	4111245..4111481	-	237	WP_012702579.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4100537..4116052	15515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10796.24 Da
• Isoelectric Point: 8.0567

>T29392 WP_012702568.1 NC_021150:c4107176-4106886 [Azotobacter vinelandii CA6]
MKSILTTDVFDRWFSGLRDRRAAVRVQARIDRAEDGNFGDCEPVGEGVSEMRIHYGPGYRVYFTQRGHELVILLAGGDKS
SQAKDIKAAIDMARHL

Nucleotide

• Length: 291 bp

>T29392 NC_021150:c4107176-4106886 [Azotobacter vinelandii CA6]
ATGAAAAGCATCCTCACCACGGACGTGTTCGACCGCTGGTTCTCCGGCCTGCGGGACAGGCGGGCGGCAGTGCGAGTACA
GGCCCGCATTGACCGTGCCGAAGATGGCAACTTCGGCGATTGCGAGCCGGTCGGCGAAGGCGTGTCGGAGATGCGCATCC
ACTACGGGCCGGGCTATCGCGTGTATTTCACGCAACGCGGGCACGAGTTGGTCATCCTGCTGGCCGGTGGCGACAAGTCC
AGCCAAGCGAAAGACATCAAGGCCGCTATCGACATGGCGCGGCATCTGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10922.51 Da
• Isoelectric Point: 6.4795

>AT29392 WP_012702567.1 NC_021150:c4106876-4106568 [Azotobacter vinelandii CA6]
MTTVKLRKWDSAEHLKTDEDMALYLEACIEEAGDDAAFIAKALGTIARAKGMAQLARDTGLGRESLYKALSGEGNPSFAT
ILKVIQALGLRLHVEAGAAHTH

Nucleotide

• Length: 309 bp

>AT29392 NC_021150:c4106876-4106568 [Azotobacter vinelandii CA6]
ATGACTACCGTCAAACTGCGCAAGTGGGATAGCGCCGAACACCTCAAGACCGATGAGGACATGGCGCTTTATCTTGAGGC
TTGCATCGAGGAGGCTGGCGACGATGCGGCGTTCATCGCCAAGGCTCTCGGCACCATCGCCCGCGCCAAGGGCATGGCCC
AATTGGCACGAGACACCGGCCTCGGGCGCGAAAGTCTGTACAAGGCGCTGTCCGGCGAGGGCAACCCCAGCTTCGCCACC
ATCCTCAAGGTGATACAGGCGCTCGGCCTCCGGCTTCACGTTGAAGCCGGTGCGGCGCACACTCACTGA

Structures

Toxin

• AlphaFold DB: M9YR94