Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 5595524..5596563 Replicon chromosome
Accession NZ_LT963402
Organism Pseudomonas syringae pv. avii isolate CFBP3846

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A2K4W0T2
Locus tag C6H38_RS26725 Protein ID WP_060413860.1
Coordinates 5595988..5596563 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2G9KX16
Locus tag C6H38_RS26720 Protein ID WP_005737550.1
Coordinates 5595524..5595991 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H38_RS26695 5591861..5592112 + 252 Protein_4946 phosphoribulokinase -
C6H38_RS26700 5592437..5593459 + 1023 WP_002551322.1 IS21-like element ISPsy4 family transposase -
C6H38_RS26705 5593456..5594265 + 810 WP_005741073.1 IS21-like element ISPsy4 family helper ATPase IstB -
C6H38_RS26710 5594314..5594703 - 390 Protein_4949 hypothetical protein -
C6H38_RS32575 5594749..5595249 - 501 WP_060413859.1 hypothetical protein -
C6H38_RS26720 5595524..5595991 + 468 WP_005737550.1 helix-turn-helix domain-containing protein Antitoxin
C6H38_RS26725 5595988..5596563 + 576 WP_060413860.1 PIN domain-containing protein Toxin
C6H38_RS26730 5596744..5596995 + 252 WP_054089800.1 helix-turn-helix domain-containing protein -
C6H38_RS26735 5596995..5597180 + 186 Protein_4954 HipA N-terminal domain-containing protein -
C6H38_RS26740 5597979..5598548 + 570 Protein_4955 site-specific integrase -
C6H38_RS26745 5598618..5598893 + 276 WP_025168237.1 hypothetical protein -
C6H38_RS26750 5598940..5599227 + 288 WP_060413862.1 hypothetical protein -
C6H38_RS26755 5599208..5600763 + 1556 WP_103724633.1 IS3-like element IS1240 family transposase -
C6H38_RS26760 5600837..5601400 + 564 WP_060412766.1 DUF2971 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5586993..5602703 15710
- inside IScluster/Tn - - 5592437..5603869 11432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21958.14 Da        Isoelectric Point: 5.5934

>T293913 WP_060413860.1 NZ_LT963402:5595988-5596563 [Pseudomonas syringae pv. avii]
MRHSPFTAFYDANVLYPAPLRDFLMHLALTGVYRARWSSQIHDEWKRNLLINRPELTREQLDRTSALMDKAVPDGLVSDY
QSLIEGLKLPDADDRHVLAAAIKCNASVIVTFNLKDFPKDILDTFDMEPLHPDDFIADLWDLDKAAVLEAAQRQRASLRN
PAHSARQYLDKLLQQKLPETVKLLSDFEFLI

Download         Length: 576 bp

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17233.75 Da        Isoelectric Point: 5.2911

>AT293913 WP_005737550.1 NZ_LT963402:5595524-5595991 [Pseudomonas syringae pv. avii]
MSSADATRTVLPGEKEIAAAVESSRQLAAFLTTKCDTQRIELVDETQQREVVELPMFALRLLGEILSELALGNAVKVVPI
HAELTTQEAADMLNVSRPHLVKMLDQGMLPHTKTGRHRRVKFADLMSYKQQRDQASREAMDELAAQAQELGMGYE

Download         Length: 468 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4W0T2

Antitoxin

Source ID Structure
AlphaFold DB A0A2G9KX16

References