Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5363226..5364955 Replicon chromosome
Accession NZ_LT963402
Organism Pseudomonas syringae pv. avii isolate CFBP3846

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2V0QPP4
Locus tag C6H38_RS25465 Protein ID WP_003379114.1
Coordinates 5363226..5363870 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A2K4W045
Locus tag C6H38_RS25470 Protein ID WP_024698668.1
Coordinates 5363867..5364955 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H38_RS25440 5358278..5359087 + 810 WP_060411913.1 zinc-dependent peptidase -
C6H38_RS25445 5359198..5359725 + 528 WP_005614429.1 inorganic diphosphatase -
C6H38_RS25450 5360084..5360815 + 732 WP_005770134.1 helix-turn-helix transcriptional regulator -
C6H38_RS25455 5361117..5362238 - 1122 WP_060411912.1 DUF91 domain-containing protein -
C6H38_RS25460 5362648..5363229 + 582 WP_060411911.1 DUF4433 domain-containing protein -
C6H38_RS25465 5363226..5363870 + 645 WP_003379114.1 DUF4433 domain-containing protein Toxin
C6H38_RS25470 5363867..5364955 + 1089 WP_024698668.1 macro domain-containing protein Antitoxin
C6H38_RS25475 5365038..5365271 - 234 Protein_4723 helix-turn-helix domain-containing protein -
C6H38_RS32765 5365261..5365437 - 177 WP_010201210.1 hypothetical protein -
C6H38_RS25485 5365489..5365839 - 351 WP_080439103.1 hypothetical protein -
C6H38_RS25490 5365969..5366241 + 273 WP_007245505.1 helix-turn-helix domain-containing protein -
C6H38_RS25500 5366549..5367358 - 810 WP_005741073.1 IS21-like element ISPsy4 family helper ATPase IstB -
C6H38_RS25505 5367355..5368377 - 1023 WP_002551322.1 IS21-like element ISPsy4 family transposase -
C6H38_RS25510 5368463..5369083 - 621 Protein_4729 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24813.38 Da        Isoelectric Point: 6.4920

>T293912 WP_003379114.1 NZ_LT963402:5363226-5363870 [Pseudomonas syringae pv. avii]
MIYSSLNPEKALIWRIVHRDNLPWILDNGLHCASSYMQAPQYVNIGNADLIDKRRNRSVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLPRIEQLGLAFVFTNAHAYPDWTDYYSDLVNLDQIDWPIIQKRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40593.60 Da        Isoelectric Point: 6.3998

>AT293912 WP_024698668.1 NZ_LT963402:5363867-5364955 [Pseudomonas syringae pv. avii]
MIRFTQGNLLEADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNHVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGINDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLCKSGVSPTVPTVREA
LKHWDGGAGAAARKSRLFDDKAIDIALKRLTTSSLSPEMAAS

Download         Length: 1089 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2K4W045

References