Detailed information of TA system TA029391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4031093..4031865
• Replicon: chromosome
• Accession: NC_021150
• Organism: Azotobacter vinelandii CA6

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: M9YTF4
• Locus tag: AVCA6_RS18130
• Protein ID: WP_012702494.1
• Coordinates: 4031093..4031575 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: M9YR46
• Locus tag: AVCA6_RS18135
• Protein ID: WP_012702495.1
• Coordinates: 4031572..4031865 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVCA6_RS18110	4027072..4027944	+	873	WP_012702490.1	DMT family transporter	-
AVCA6_RS18115	4028033..4029403	+	1371	WP_012702491.1	exodeoxyribonuclease VII large subunit	-
AVCA6_RS18120	4029408..4030172	+	765	WP_012702492.1	M15 family metallopeptidase	-
AVCA6_RS18125	4030194..4031018	+	825	WP_012702493.1	peptidoglycan DD-metalloendopeptidase family protein	-
AVCA6_RS18130	4031093..4031575	-	483	WP_012702494.1	GNAT family N-acetyltransferase	Toxin
AVCA6_RS18135	4031572..4031865	-	294	WP_012702495.1	DUF1778 domain-containing protein	Antitoxin
AVCA6_RS18140	4032058..4033503	+	1446	WP_012702496.1	TldD/PmbA family protein	-
AVCA6_RS18145	4033500..4034819	+	1320	WP_012702497.1	Zn-dependent protease	-
AVCA6_RS18150	4034858..4035118	-	261	WP_012702498.1	hypothetical protein	-
AVCA6_RS18155	4035745..4036470	+	726	WP_012702500.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-89)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17182.88 Da
• Isoelectric Point: 9.8643

>T29391 WP_012702494.1 NC_021150:c4031575-4031093 [Azotobacter vinelandii CA6]
MRLGAPEPLSERHDVESFSSGSEGLDLWLKRRALKNRLSGASRTFVACEGRRVLAYYALASGVIAVDRAPGRFCRNMPDP
VPVVVLGRLAVDLSLQGQGLGRALVRDAALRILQAADAIGIRGMLVHALSSDAKAFHERVGFEPSPLEPITTLADLRAGL

Nucleotide

• Length: 483 bp

>T29391 NC_021150:c4031575-4031093 [Azotobacter vinelandii CA6]
ATGAGGCTCGGCGCTCCCGAGCCCCTGTCGGAAAGGCACGATGTCGAATCCTTCTCGTCCGGCAGCGAGGGCCTGGACCT
CTGGCTGAAGCGGCGTGCGCTGAAGAACCGGCTTTCCGGTGCGTCGCGTACCTTCGTCGCCTGCGAGGGGCGTAGGGTTC
TGGCCTACTACGCGCTGGCATCGGGTGTCATCGCCGTCGATCGGGCGCCGGGACGATTTTGTCGCAACATGCCGGACCCG
GTTCCGGTCGTCGTCCTCGGGCGGCTGGCCGTGGATCTTTCCCTGCAGGGGCAGGGCCTCGGACGGGCCCTGGTGCGCGA
CGCCGCGCTGCGCATCCTGCAGGCGGCGGATGCGATCGGCATTCGCGGCATGCTCGTCCACGCCCTGTCCTCCGATGCGA
AGGCGTTCCACGAGCGGGTCGGTTTCGAGCCGTCGCCGCTGGAGCCGATAACGACCCTGGCCGACTTGAGAGCCGGGCTT
TGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10939.37 Da
• Isoelectric Point: 6.3887

>AT29391 WP_012702495.1 NC_021150:c4031865-4031572 [Azotobacter vinelandii CA6]
MSISNPAPARRETLNIRIKPEERSLIDRAAQVKGKNRTDFILDAARTAAEEALLDRAFIAASPEAYAEFLAARLDRPPQP
NERLRKTLQTPAPWDEE

Nucleotide

• Length: 294 bp

>AT29391 NC_021150:c4031865-4031572 [Azotobacter vinelandii CA6]
ATGTCTATATCCAACCCTGCGCCGGCCAGGCGCGAAACCCTGAATATCCGCATCAAGCCCGAAGAGCGCAGCCTGATCGA
CCGTGCCGCCCAGGTGAAAGGCAAGAACCGCACGGATTTCATCCTGGATGCGGCGCGCACGGCGGCCGAGGAGGCCCTGC
TCGACCGGGCCTTCATCGCCGCCAGCCCGGAAGCCTACGCCGAGTTCCTGGCGGCCCGGCTGGACAGGCCGCCGCAGCCC
AATGAACGCTTGCGCAAGACGCTGCAGACCCCGGCACCCTGGGACGAGGAATGA

Structures

Toxin

• AlphaFold DB: M9YTF4

Antitoxin

• AlphaFold DB: M9YR46