Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 30543..31216 Replicon plasmid PP2
Accession NZ_LT963397
Organism Pseudomonas cerasi isolate PL963

Toxin (Protein)


Gene name vapC Uniprot ID A0A2K4W2P1
Locus tag C6H34_RS28195 Protein ID WP_060402639.1
Coordinates 30797..31216 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag C6H34_RS28190 Protein ID WP_003381346.1
Coordinates 30543..30800 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H34_RS28160 27404..27769 - 366 Protein_29 MobA/MobL family protein -
C6H34_RS28165 27808..28080 - 273 WP_015060672.1 hypothetical protein -
C6H34_RS30835 28234..28491 + 258 WP_157893878.1 hypothetical protein -
C6H34_RS28175 28799..29080 + 282 WP_060402791.1 hypothetical protein -
C6H34_RS28180 29061..30290 + 1230 WP_065348575.1 IS91 family transposase -
C6H34_RS28190 30543..30800 + 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
C6H34_RS28195 30797..31216 + 420 WP_060402639.1 type II toxin-antitoxin system VapC family toxin Toxin
C6H34_RS28200 31257..31889 + 633 WP_103750722.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..144075 144075
- flank IS/Tn - - 29082..30290 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15164.46 Da        Isoelectric Point: 4.5482

>T293898 WP_060402639.1 NZ_LT963397:30797-31216 [Pseudomonas cerasi]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKYLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT293898 WP_003381346.1 NZ_LT963397:30543-30800 [Pseudomonas cerasi]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4W2P1

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References