Detailed information of TA system TA029389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3703858..3704387
• Replicon: chromosome
• Accession: NC_021150
• Organism: Azotobacter vinelandii CA6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M9YLU7
• Locus tag: AVCA6_RS16555
• Protein ID: WP_012702163.1
• Coordinates: 3703858..3704181 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AVCA6_RS16560
• Protein ID: WP_041807346.1
• Coordinates: 3704178..3704387 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVCA6_RS16530	3699058..3699990	+	933	WP_012702159.1	TIGR03756 family integrating conjugative element protein	-
AVCA6_RS16535	3700013..3701377	+	1365	WP_012702160.1	integrating conjugative element protein	-
AVCA6_RS16540	3701384..3701740	+	357	WP_012702161.1	hypothetical protein	-
AVCA6_RS16545	3701737..3703302	+	1566	WP_012702162.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
AVCA6_RS16550	3703320..3703625	-	306	WP_041807344.1	hypothetical protein	-
AVCA6_RS25110	3703678..3703851	-	174	WP_081434905.1	hypothetical protein	-
AVCA6_RS16555	3703858..3704181	-	324	WP_012702163.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVCA6_RS16560	3704178..3704387	-	210	WP_041807346.1	antitoxin MazE family protein	Antitoxin
AVCA6_RS16565	3704581..3705183	+	603	WP_012702164.1	DUF4852 domain-containing protein	-
AVCA6_RS16570	3705296..3705535	-	240	WP_175556057.1	Arc family DNA-binding protein	-
AVCA6_RS16575	3705885..3706918	+	1034	Protein_3321	IS630 family transposase	-
AVCA6_RS25120	3707335..3707973	+	639	WP_012702167.1	hypothetical protein	-
AVCA6_RS16580	3708195..3708902	-	708	WP_012702168.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3617323..3712021	94698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (5-68)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11433.43 Da
• Isoelectric Point: 9.7779

>T29389 WP_012702163.1 NC_021150:c3704181-3703858 [Azotobacter vinelandii CA6]
MNRGAVVVVALQGAYGKPRPALVIQSDLFSALPSVTILPITSDLREAPLYRIDIEAAPRTGLHKPSQIMVDKPQTIPREK
VGEVIGSVNAETMLAVSRSLTVFLGIA

Nucleotide

• Length: 324 bp

>T29389 NC_021150:c3704181-3703858 [Azotobacter vinelandii CA6]
ATGAATCGAGGTGCCGTGGTGGTGGTCGCCTTGCAGGGCGCCTACGGAAAGCCTCGCCCGGCGTTGGTCATCCAGTCCGA
TCTGTTCTCGGCGTTGCCTTCCGTGACCATTCTTCCTATCACGAGCGATTTGCGGGAGGCGCCGCTTTATCGCATCGACA
TCGAGGCCGCGCCGAGAACCGGGTTGCACAAGCCATCACAGATCATGGTGGACAAGCCGCAAACGATCCCCCGCGAGAAA
GTGGGCGAGGTGATCGGCTCGGTCAACGCAGAAACCATGCTGGCCGTGAGTCGGTCGCTAACCGTGTTTCTCGGTATTGC
TTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8061.07 Da
• Isoelectric Point: 5.1502

>AT29389 WP_041807346.1 NC_021150:c3704387-3704178 [Azotobacter vinelandii CA6]
MQTVRERVRKRREALRAAGLRPVQIWVPDTRAPGFAEEARRQCLLVQNDPQEKESLEWLESAADTEGWE

Nucleotide

• Length: 210 bp

>AT29389 NC_021150:c3704387-3704178 [Azotobacter vinelandii CA6]
ATGCAGACCGTGCGTGAGCGGGTCAGGAAGCGGCGCGAGGCGCTGCGGGCTGCTGGGTTGCGCCCGGTGCAAATCTGGGT
GCCGGACACCCGTGCTCCCGGCTTTGCGGAAGAGGCGAGGCGGCAGTGCCTTCTCGTACAGAATGATCCGCAAGAGAAAG
AAAGTCTGGAGTGGCTGGAAAGCGCGGCTGACACAGAGGGGTGGGAATGA

Structures

Toxin

• AlphaFold DB: M9YLU7