293886

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1044802..1045361 Replicon chromosome
Accession NZ_LT962947
Organism Brucella melitensis isolate

Toxin (Protein)


Gene name brnT Uniprot ID Q8YH01
Locus tag C0M00_RS05350 Protein ID WP_004683741.1
Coordinates 1044802..1045047 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag C0M00_RS05355 Protein ID WP_004683740.1
Coordinates 1045047..1045361 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0M00_RS05325 1040649..1042100 - 1452 WP_006144496.1 hypothetical protein -
C0M00_RS05330 1042430..1043203 - 774 WP_002967634.1 hypothetical protein -
C0M00_RS05335 1043204..1043961 - 758 Protein_982 IS5 family transposase -
C0M00_RS05345 1044031..1044669 - 639 Protein_983 tRNA-guanine transglycosylase -
C0M00_RS05350 1044802..1045047 + 246 WP_004683741.1 BrnT family toxin Toxin
C0M00_RS05355 1045047..1045361 + 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
C0M00_RS05365 1045709..1045879 + 171 WP_002964095.1 DUF1508 domain-containing protein -
C0M00_RS05370 1046227..1046937 - 711 WP_004683739.1 porin family protein -
C0M00_RS05375 1047172..1047759 - 588 WP_004683738.1 hypothetical protein -
C0M00_RS05380 1047782..1048057 - 276 WP_002964092.1 hypothetical protein -
C0M00_RS05385 1048054..1048284 - 231 WP_002964091.1 hypothetical protein -
C0M00_RS05390 1048281..1048985 - 705 WP_006137281.1 hypothetical protein -
C0M00_RS05395 1049035..1049238 + 204 WP_002964088.1 hypothetical protein -
C0M00_RS05400 1049235..1049450 + 216 WP_005968947.1 hypothetical protein -
C0M00_RS05405 1049453..1049656 + 204 WP_004683737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wbdA / wboA 1037535..1051428 13893
- flank IS/Tn - - 1043593..1043961 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9196.83 Da        Isoelectric Point: 10.3158

>T293886 WP_004683741.1 NZ_LT962947:1044802-1045047 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVFFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT293886 WP_004683740.1 NZ_LT962947:1045047-1045361 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8YH01

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References